Thermo Fisher, 4373872, AmpFLSTR™ MiniFiler™ PCR Amplification Kit

AmpFLSTR™ MiniFiler™ PCR Amplification Kit is used to recover STR information from degraded, compromised or limited forensic DNA samples. The kit uses a miniSTR design that shortens the amplified regions for eight difficult-to-amplify loci also represented in established AmpFLSTR systems, improving the chance of obtaining interpretable results when longer amplicons are lost through DNA degradation. This 100-reaction configuration uses 25 µL amplification reactions and includes MiniFiler Master Mix, MiniFiler Primer Set, DNA Control 007 and a MiniFiler Allelic Ladder. The multiplex is intended for forensic or paternity applications rather than diagnostic testing. It is particularly useful for re-analysis of older evidence and other challenging specimens where conventional STR assays may produce partial profiles. Amplified fragments are separated and detected using compatible capillary-electrophoresis genetic analyzers.