Thermo Fisher, 4427012, TaqMan™ Pri-miRNA Assay, S (360 reactions), made to order

Quantity: S (360 reactions), made to order
Unit Size: Each
TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold-standard assay performance and data quality for pri-miRNA studies.• Highly specific—quantitiate miRNA transcription from a single genomic locus• Fast, simple, and scalable—two-step RT-qPCR provides high-quality results• A new perspective—measure miRNA expression at the gene levelTaqMan Pri-miRNA Assays apply the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan genomic assays are renowned to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.Design and selectionSince all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan Pri-miRNA Assay and TaqMan MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.Pre-formulated assay (20X; 60X mix)• 2 unlabeled PCR primers (900 nM each final 1X reaction concentration)• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X reaction concentration)