Thermo Fisher, 4440040, TaqMan™ Universal Master Mix II, no UNG, 1 x 5 mL

Name: TaqMan™ Universal Master Mix II, no UNG, 1 x 5 mL
Brand: Thermo Fisher
SKU: 4440040

Quantity: 1 x 5 mL, Unit Size: Each

Catalog Number: 4440040

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Category

qPCR Master Mixes

Pack Size1 x 5 mL

TagsPCR Equipment and Supplies PCR Supplies Real Time PCR Products Real Time PCR Master Mixes

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 x 5 mL
Unit Size: Each
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)• dNTPs (with dUTP)• ROX Passive Reference• Optimized buffer componentsNote: This TaqMan Universal Master Mix II does not contain uracil-N-glycoslyase (UNG).TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service or with pre-optimized assays, such as:• TaqMan gene expression assays• TaqMan microRNA assays• TaqMan drug metabolism genotyping assays• TaqMan SNP genotyping AssaysFor RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.Alternative product: Try TaqMan Fast Advanced Master Mix (Cat. No. 4444963), our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

Specifications

General

Fidelity (vs. Taq)	2 X
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye	ROX (Pre-mixed)
Polymerase	AmpliTaq Gold DNA Polymerase
Product Line	TaqMan
Product Type	Universal Master Mix II
Purity or Quality Grade	UP (Ultra Pure)
Quantity	1 x 5 mL
Sample Type	DNA (Genomic), cDNA
Shipping Condition	Wet Ice
Volume	5 mL
Concentration	2X
Detection Method	Primer-probe
For Use With (Application)	Gene Expression
GC-Rich PCR Performance	High
PCR Method	qPCR
Reaction Speed	Standard
Unit Size	Each

FAQs

Question: What concentration is the TaqMan Universal Master Mix II supplied at?

Answer:It is supplied as a 2X master mix.

Question: Does this formulation contain UNG?

Answer:No. This version does not contain uracil-N-glycosylase.

Question: How many 50 µL reactions can one 5 mL bottle support?

Answer:The 5 mL bottle supports approximately 200 reactions at a 50 µL reaction volume.

Application Note

TaqMan™ Universal Master Mix II, no UNG is used for probe-based real-time PCR amplification and detection of DNA or cDNA targets. The ready-to-use 2X master mix contains AmpliTaq Gold DNA Polymerase, dNTPs including dUTP, ROX passive reference dye and optimized buffer components. Primers, TaqMan probes, template and water are added separately. This formulation does not contain uracil-N-glycosylase, making it appropriate for workflows that do not require enzymatic carryover-contamination control. The 5 mL bottle supports approximately 200 reactions at a 50 µL reaction volume. Applications include gene-expression quantification, microRNA assays and SNP or drug-metabolism genotyping using compatible real-time PCR instruments. The premixed formulation reduces setup variability and simplifies preparation of multiple qPCR reactions.

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