Description
Includes: Assay Kit and Sample Preparation
Unit Size: Each
Features of the resDNASEQ Quantitative Pichia pastoris DNA Kit include:• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology• Consistent performance across the expected range of DNA fragment sizes• Includes master mix, TaqMan™ primer/probe mix, and Pichia pastoris genomic DNA standardThis kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.The resDNASEQ™ Quantitative Pichia pastoris DNA Kit is designed to provide highly sensitive detection of Pichia pastoris DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan™ real-time qPCR technology allows you to test samples with variable levels of Pichia pastoris DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.Easy to useWith the resDNASEQ™ Quantitative Pichia pastoris DNA Kit you can perform qPCR analysis to compare the amount of Pichia pastoris DNA in your test samples to a standard curve generated with known amounts of purified Pichia pastoris genomic DNA standard. The resDNASEQ™ kit includes almost everything you need to carry out these steps, from TaqMan™ primer/probe mix to Pichia pastoris genomic DNA standard, as well as step-by-step instructions.Specific to Pichia pastoris DNA, no cross-reactivity with unrelated DNAThe target of the assay is highly specific—it only detects a Pichia pastoris-specific region of a multicopy genetic element. We selected this region using extensive bioinformatic analysis of multiple related and unrelated species.Consistent performance even with fragmented DNAFor accurate quantitation of residual Pichia pastoris DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ™ system you get consistent assay performance across a range of DNA fragment sizes.
