Thermo Fisher, 450640, TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells, 25 Reactions

Quantity: 25 Reactions
Unit Size: Each
Includes the pCR™II-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable cloning method, featuring a 5-minute cloning reaction and 3-step protocol, and yielding up to 95% recombinants. Resistant to Kanamycin (KanR) andZeocin™ (ZeoR)Kit Options: TOPO TA Cloning Dual Promoter KitsTOPO TA Cloning Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:
• General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40)
• High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K4660-01, K4660-40)
• General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40)
• Fast growth: Mach1™ -T1R chemically competentE. coli(Cat. No. K4610-20)
• Repressor/induction needs: TOP10F' cells (Cat. Nos. K4650-01, K4650-40)
• Provide your own cells (Cat. Nos. 450640 and 452640)
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient restriction sites, includingEcoRI, flanking your insert for subsequent excision or subcloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinantsCloning, PCR Cloning