Thermo Fisher, 452640, TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells, 10 Reactions

Name: TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells, 10 Reactions
Brand: Thermo Fisher
SKU: 452640

Quantity: 10 Reactions, Unit Size: Each

Catalog Number: 452640

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Category

Competent Cells

Pack Size10 reactions

TagsBiochemical Reagents DNA Reagents PCR Cloning Kits

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 Reactions
Unit Size: Each
Includes the pCR™II-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable cloning method, featuring a 5-minute cloning reaction and 3-step protocol, and yielding up to 95% recombinants. Resistant to Kanamycin (KanR) andZeocin™ (ZeoR)Kit Options: TOPO TA Cloning Dual Promoter KitsTOPO TA Cloning Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:
• General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40)
• High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K4660-01, K4660-40)
• General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40)
• Fast growth: Mach1™ -T1R chemically competentE. coli(Cat. No. K4610-20)
• Repressor/induction needs: TOP10F' cells (Cat. Nos. K4650-01, K4650-40)
• Provide your own cells (Cat. Nos. 450640 and 452640)
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient restriction sites, includingEcoRI, flanking your insert for subsequent excision or subcloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinantsCloning, PCR Cloning

Specifications

General

Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Cloning Method	TOPO™-TA
For Use With (Application)	Cloning and subsequent in vitro transcription
Includes	Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water
Product Line	TOPO
Product Type	TA Cloning Kit
Promoter	T7, SP6
Quantity	10 Reactions
Shelf Life	All reagents are stable for 6 months when properly stored.
Vector	TOPO-TA Vectors
Format	Kit
Unit Size	Each

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Thermo Fisher, 452640, TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells, 10 Reactions

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