Thermo Fisher, 60111-104, SMART Digest™ ImmunoAffinity (IA) Protein A Kits

Description: Magnetic
Unit Size: Each
Solving the challenges of low-level quantitation in complex matrices The need to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging. As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility. Immunocapture and Protein Digestion in a Single Well These IA kits deliver a process that is:
• Fast
• Simple
• Highly reproducible
• Sensitive Unique Design Reduces Workflow to Hours In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.