Thermo Fisher, 65-0853-39, eBioscience™ Calcein AM Viability Dye (UltraPure Grade), 1 mg

Name: eBioscience™ Calcein AM Viability Dye (UltraPure Grade), 1 mg
Brand: Thermo Fisher
SKU: 65-0853-39

Quantity: 1 mg, Unit Size: Each

Catalog Number: 65-0853-39

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Category

Cell Lysis Buffers

Pack Size1 mg

TagsCell Analysis Products Cell Based Assays Cell Function Reagents and Kits

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 mg
Unit Size: Each
• Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye
• Apoptotic and dead cells with compromised cell membranes do not retain Calcein
• Calcein is optimally excited at 495 nm and has a peak emission of 515 nm
• For flow cytometric analysis, it can be excited off the blue laser line (488 nm) and detected using filters for FITC/Alexa Fluor™ 488 (530/30)
• For multicolor flow cytometry, concentrations in the range of 5 to 20 nM are recommended to ensure that compensation can be performed successfully
• Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells
• If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat. 65-0855) or Calcein Violet 450 AM (cat. 65-0854)
• Calcein AM should be reconstituted in high-quality, freshly opened DMSO
• Once reconstituted, it should be stored at -20°C with dessicant and used within a short period of time (avoid freeze-thawing)
• Purity: >95% (determined by HPLC)

Specifications

General

Detection Method	Fluorescence
Quantity	1 mg
Color	Green
Emission	495/515
For Use With (Application)	Viability Assay
For Use With (Equipment)	Fluorescence Microscope
Product Line	eBioscience
Product Type	Dye
Unit Size	Each

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Thermo Fisher, 65-0853-39, eBioscience™ Calcein AM Viability Dye (UltraPure Grade), 1 mg

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General

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