Description
Quantity: 250 Devices
Unit Size: Each
Features of Slide-A-Lyzer MINI Dialysis Devices, 2K MWCO: • Flexible format—enables user to dialyze a single sample (tube) or multiple samples (25 array) • Excellent sample recovery—low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems • One-step protocol—does not require laborious procedures or expensive equipment • 100% leak-tested—innovative design does not permit 'wicking' that can occur in homemade devices • Time saving—performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialyzing small-volume samples of nucleic acids and proteins without undue sample loss. They are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use. The 0.1-mL MINI dialysis devices are designed to be capped (caps included) and inserted into standard 1.5 mL microcentrifuge tubes. Alternatively, as many as 25 MINI devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple samples inside a large beaker. Using the 0.1-mL MINI device, 100 μL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes. As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume; temperature and agitation rates; the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes; and the pH, ionic strength, and solvent characteristic of the buffers being used.
