Thermo Fisher, 72400021, RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

Name: RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES
Brand: Thermo Fisher
SKU: 72400021

Quantity: 500 mL, Unit Size: Each, Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

Catalog Number: 72400021

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Category

Classical Cell Culture Media

Pack Size500 mL

TagsCell Culture Media Supplements and Reagents Classical and Basal Cell Culture Media RPMI Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 mL
Unit Size: Each
RPMI 1640 is modified as follows:With
• GlutaMAX™
• Phenol Red
• HEPESThe complete formulation is available.Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B_12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. Gibco™ RPMI with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.cGMP Manufacturing and Quality SystemGibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ RPMI 1640 product made in our Grand Island facility (72400-120). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO_2 environment to maintain physiological pH.

Specifications

General

Cell Type	Mammalian Cells
Concentration	1 X
Culture Environment	CO<sub>2</sub>
Osmolality	270 - 310 mOsm/kg
Product Line	Gibco, GlutaMAX
Product Type	RPMI 1640
Quantity	500 mL
Shelf Life	12 Months
Shipping Condition	Room Temperature
Classification	Animal Origin-free
Culture Type	Mammalian Cell Culture
Form	Liquid
Serum Level	Standard Serum Supplementation
Sterility	Sterile-filtered
With Additives	HEPES
Without Additives	No Sodium Pyruvate
pH	7 to 7.4
Unit Size	Each

FAQs

Question: Does this RPMI 1640 formulation contain GlutaMAX and HEPES?

Answer:Yes. The medium contains GlutaMAX, HEPES, phenol red and sodium bicarbonate.

Question: Does the 500 mL medium already contain fetal bovine serum?

Answer:No. It is a basal medium, and serum or other supplements are added for the specific cell line.

Question: How should the medium be stored?

Answer:Store the liquid medium at 2–8°C and protect it from light.

Application Note

Gibco™ RPMI 1640 Medium with GlutaMAX™ and HEPES is a ready-to-use basal medium for routine mammalian cell culture. The 500 mL sterile-filtered liquid formulation includes stable GlutaMAX dipeptide in place of conventional L-glutamine, HEPES for additional buffering capacity and phenol red as a visual pH indicator. RPMI 1640 supports diverse suspension and adherent cell models, including lymphoid cell lines and peripheral blood mononuclear cells. As a basal medium, it normally requires supplementation with serum or other growth factors suited to the specific cell line. The sodium bicarbonate buffering system still requires an appropriate carbon-dioxide incubator atmosphere for physiological pH control. The formulation does not include sodium pyruvate. Store at 2–8°C and protect from light to reduce degradation of light-sensitive medium components. Choose the culture conditions and supplement concentration using validated procedures for the target cell type.

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