Description
Quantity: 10 mg
Unit Size: Each
Purified, carrier-grade BSA is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Several antigen molecules can be conjugated to each BSA molecule to increase their immunogenicity and likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Select from among several package sizes of activated BSA protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns. Highlights:
• Convenient packages– choose stabilized, lyophilized Maleimide-Activated BSA or complete immunogen conjugation kit
• Optimized activation– specifications require that each lot is activated with 15 to 20 moles of sulhydryl-reactive maleimide per mole of BSA, ensuring consistently high levels of conjugation
• Sulfhydryl-reactive– engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production
• Simple, rapid procedure– complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals
• High-quality carrier protein– pure BSA (Fraction V) is activated using high-quality Thermo Scientific Sulfo-SMCC crosslinker and formulated to ensure high stability and consistent performance
• Immunogenic and soluble– moderately immunogenic and highly soluble, BSA is useful for preparation of the primary immunogen or as an irrelevant hapten-carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against the hapten Includes:Kit contains stabilized, lyophilized Maleimide-Activated BSA (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization
