Thermo Fisher, 78999, Y-PER™ Plus Dialyzable Yeast Protein Extraction Reagent

Quantity: 500 mL
Unit Size: Each
The proprietary formulation of cell lysis buffer facilitates protein extraction from S. cerevisiae and S. pastoris yeast cell cultures. Glass bed disruption is not necessary; protein extraction is achieved by a 20-minute incubation in the reagent at room temperature. Unlike other cell lysis buffers, the Y-PER-Plus Reagent uses a detergent component that is fully dialyzable, allowing complete exchange of the extracted protein into any buffer required by downstream techniques. Highlights:
• Stable formulation designed for room temperature storage
• Yields protein extract with simple 20-minute hands-off incubation
• Requires no rigorous mechanical disruption or lengthy enzymatic treatments
• Tris-based buffer has a very low ionic strength and does not include sodium chloride
• Simple formulation contains no reducing agents or chelating agents
• Fully dialyzable formulation provides complete control over downstream applications
• Compatible with both the Thermo Scientific Pierce™ BCA and Coomassie Plus™ Protein Assays