Thermo Fisher, 810299010, CaptureSelect™ VII Select Leakage ELISA

CaptureSelect™ VII Select Leakage ELISA is designed for measuring VIISelect affinity-ligand leakage during purification of human coagulation Factor VII using VIISelect affinity resin. The test detects ligand that has detached from the chromatography medium and co-eluted with the purified protein. It uses an ELISA workflow with a goat IgG coating reagent directed against the VIISelect ligand, a VIISelect ligand standard and a biotinylated detection reagent. The product provides ten assays, allowing leakage analysis during process development as well as routine testing of process intermediates and final purified material. The assay supports measurement of leached ligand at concentrations of 1 ng/mL and above and is read using a microplate reader. The ligand-specific reagents distinguish this method from general protein measurements or assays for unrelated Protein A ligands. Use the standards and supplied immunoassay reagents to establish the response required for quantitation of residual VIISelect ligand in relevant process samples. The kit is for research use and is not intended for diagnostic procedures. Store the components frozen at −5 to −30 °C. The ten-assay format is suited to repeated leakage checks during affinity-process optimization. The assay format is based on a target-specific standard curve and antibody capture/detection reagents. It is therefore appropriate for monitoring the actual VIISelect ligand rather than simply measuring total residual protein in the Factor VII product.