Description
Quantity: 1 Kit
Unit Size: Each
The complete Subcellular Protein Fractionation Kit for Tissue includes a unique 250μm tissue strainer to facilitate rapid separation of cell suspension from tissue debris after homogenization. The optimized reagents enable subsequent stepwise extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from the recovered cells with less than 15% cross-contamination. Tissue extracts obtained with this subcellular fractionation kit are compatible with a variety of downstream applications, including Western blotting, protein quantitation, electrophoretic mobility shift, reporter-gene and enzyme-activity assays. Highlights:
• Optimizedformulations and protocols specific for fractionation of tissue
• Completeobtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit
• Time efficientextract functional protein fractions without ultracentrifugation in approximately 2 hours
• Convenientunique tissue strainer allows rapid removal of tissue debris
• Compatibleuse extracts for downstream applications such as protein assays, Western blotting, gel-shift assays and enzyme-activity assays
• Versatileextract proteins from a variety of tissue types, including liver, heart, brain, kidney, lung and spleen Recommended for:Obtain cytoplasmic, soluble nuclear, chromatin-bound, membrane and cytoskeletal proteins from the same tissue sample; Profile disease-state versus normal tissues for proteins from the cytoplasm, nucleus, membrane and cytoskeleton; Study protein translocation in tissues