Description
Quantity: 100 mL
Unit Size: Each
Neuron cell lysates prepared with the N-PER Reagent may be used for enzymatic activity assays (e.g., Phosphatase, Kinase, ATPase Assays), immunoassays (e.g., Western blots, ELISAs, RIAs) and protein purification. The N-PER Lysis Reagent enables protein extraction to be completed in less than 30 minutes and requires mechanical disruption of tissue (e.g., Dounce Homogenization, Polytron). Typical neuronal protein yields are 70-90μg of protein per mg of brain tissue or 300μg of total protein from 10^6 primary neuronal cells. Highlights:
• Optimized– efficient extraction of total neuronal protein, including membrane proteins, from tissue or primary cultured cells
• Gentle– preserves protein function without compromising yield
• Versatile– can be supplemented with protease inhibitors, reducing or chelating agents or required cofactors
• Compatible– extracts are suitable for use with total protein, enzymatic and immunological assays and protein purification methods Recommended for:Isolate functional proteins from neuronal tissues or primary neuronal cells; Study enzymatic activity assays specific to neuronal tissue; Generate lysates from neuronal tissues for biomarker analysis
