Description
Quantity: 100 mL
Unit Size: Each
Syn-PER Reagent is used to prepare synaptosomes containing biologically active pre-and post-synaptic proteins. When used with fresh neuronal tissue or primary cultured neurons, synaptosomes prepared with the Syn-PER Reagent can be used to study synaptic transmission. The synaptosomal proteins contained in these extracts can also be used for downstream applications such as Western blotting, immunoprecipitation, enzymatic activity assays and protein-protein interaction studies. Syn-PER Synaptic Protein Extraction Reagent minimizes the degradation of phosphoproteins and is ideal for studies requiring the preservation of phosphoprotein integrity. Highlights:
• Efficient extraction– obtain up to 10μg of synaptic protein per milligram of neuronal tissue or 4μg synaptic protein per 35mm dish of primary cultured neurons (10^6cells)
• Gentle formulation– isolate viable synaptosomes, extract native synaptic proteins and preserve phosphoprotein integrity
• Fast procedure– obtain synaptosomal suspension in less than one hour
• Simple protocol– requires no ultracentrifugation steps Recommended for:Isolate functional synaptosomes to study neurotransmitter release; Extract pre- and post-synaptic proteins to identify changes in protein composition and function in synapses; Preserve and study labile or transient protein phosphorylation events associated with synapses
