Description
Quantity: 5 Column Kit
Unit Size: Each
The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale. Highlights:
• High capacity– bind up to 60mg of 6xHis-tagged protein per milliliter of resin
• Versatile– purify proteins using native or denaturing conditions
• Compatible– use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
• Flexible– available in multiple formats including bulk resin, spin columns and chromatography cartridges
• Cost-effective– reuse the same batch of resin at least five times
• Easy to use– pre-formulated buffers available for kit formats Available Formats:
• Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
• Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
• Purification Kits – complete kits in all three column sizes
• Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
• Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin Includes:Kits contain HisPur Ni-NTA Columns