Description
Quantity: 1000 U
Unit Size: Each
Pierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln▾Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design. Highlights:
• Effective– HRV 3C is designed to remove purification tags from your protein of interest
• High activity at 4°C– activity is ≥2000U/mg control protein; one unit will cleave 0.1mg control protein at 4°C after 16h
• Flexible– dual-tagged enzyme is compatible with various columns and buffers Includes:Kit contains protease, cleavage buffer and control protein. Recommended for:Removal of purification tags from fusion proteins