Thermo Fisher, 89821, B-PER™ Complete Bacterial Protein Extraction Reagent, 250 mL

Quantity: 250 mL
Unit Size: Each
• An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
• All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
• This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
• Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.Features of the B-PER Bacterial Protein Extraction Reagent:
• Detergent in Tris buffer with Lysozyme and Universal Nuclease
• Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
• Recovery of large molecular weight proteins (high MW proteins)
• Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
• More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications