Thermo Fisher, 89839, Lysosome Enrichment Kit for Tissues and Cultured Cells

Quantity: 230 mL
Unit Size: Each
These three organelle-specific kits provide the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation. Subcellular fractionation simplifies otherwise complex protein mixtures that result from whole cell lysis, thereby facilitating proteomic analysis of specific organelle targets and pathways. Each kit uses density gradient centrifugation to separate organelles from contaminating cellular structures. The isolated organelles may be used for a number of downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies. Highlights:
• Efficient and easy to use– kit reagents and gradient centrifugation separate organelles from contaminating structures
• Compatible– prepare samples for downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies
• Complete– kits contain sufficient material for 25 applications Includes:Organelle-specific Reagents A and B, Optiprep™ Cell Separation Media, and phosphate-buffered saline Requires:Ultracentrifuge, refrigerated microcentrifuge, vortex mixer, Dounce tissue grinder, sonicator