Thermo Fisher, A10192, ATTO-TAG™ FQ Derivatization Reagent (FQ; 3-(2-Furoyl)quinoline-2-Carboxaldehyde)

Name: ATTO-TAG™ FQ Derivatization Reagent (FQ; 3-(2-Furoyl)quinoline-2-Carboxaldehyde)
Brand: Thermo Fisher
SKU: A10192

Quantity: 10 mg, Unit Size: Each

Catalog Number: A10192

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Category

Fluorescent Dyes

Pack Size10 mg

TagsProtein Purification Protein Analysis Reagents Protein Purification Reagents and Kits

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 mg
Unit Size: Each
Recently, researchers described a simple method in which the ATTO-TAG FQ reagent was used to derivatize very dilute solutions (10^-8 M) of peptides. Available as standalone product or as component of an amine-derivatization kit (A-2334). Particularly well-suited to derivatization of peptides, amino acids, aminated sugars and other amine-containing molecules for detection by capillary electrophoresis with laser-induced fluorescence, as its nonfluorescent in unreacted state and reacts specifically with amines to form highly fluorescent conjugates.Anion Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis

Specifications

General

Chemical Reactivity	Amine
For Use With (Application)	Detection of primary amines
Label or Dye	ATTO-TAG FQ
Quantity	10 mg
Reactive Moiety	Aldehyde
Shipping Condition	Room Temperature
Target	Primary Amine
Form	Solid
Product Line	ATTO-TAG
Type	Derivatization Kits and Reagents
Unit Size	Each

FAQs

Question: What does ATTO-TAG FQ detect?

Answer:It is used to derivatize and detect compounds containing primary amines.

Question: Is the unreacted FQ reagent fluorescent?

Answer:No. Strong fluorescence develops after FQ reacts with primary amines.

Question: How should the reagent be stored?

Answer:Store it frozen at −5 to −30 °C and protect it from light.

Application Note

ATTO-TAG™ FQ is a derivatization reagent for ultrasensitive detection of primary amines. The unreacted reagent is nonfluorescent, while reaction with amine-containing compounds produces strongly fluorescent conjugates. This behavior supports sensitive analysis of peptides, amino acids, aminated sugars and other primary-amine targets, particularly by capillary electrophoresis with laser-induced fluorescence detection. The reagent is supplied as 10 mg of solid material and can be used as a standalone labeling reagent in workflows that are optimized for FQ chemistry. Store it frozen at −5 to −30 °C and protect it from light. Derivatization time, reagent ratio and separation conditions should be optimized for the target analyte and sample matrix.

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