Thermo Fisher, A10498, SequalPrep™ Long PCR Kit with dNTPs

Name: SequalPrep™ Long PCR Kit with dNTPs
Brand: Thermo Fisher
SKU: A10498

Quantity: 1, 000 units, Unit Size: Each

Catalog Number: A10498

Pricing

Send an RFQ.

View RFQ List

Category

DNA Polymerases

Pack Size000 units / pack

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products Long Range PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1,000 units
Unit Size: Each
• The SequalPrep™ Long PCR Kit with dNTPs provides the highest amplification success rate of any long range PCR kit
• It has been tested across multiple organisms and platforms for non-optimized amplicons up to 20 kB with little to no optimization
• Successful long range PCR with minimal optimization enables efficient PCR enrichment for a variety of applications including medical re-sequencing on next generation sequencing (NGS) platforms
• PCR amplification is the traditional method to enrich target regions of the genome for genotyping and re-sequencing applications
• To take advantage of the enhanced NGS processing capabilities, higher throughput enrichment methods have become necessary
• This kit can be used upstream of any NGS platform or for other genotyping applications
• When combined with the SequalPrep™ Normalization Plate the SequalPrep™ (Sequence Equalization Sample Preparation) System provides a viable and efficient enrichment method to meet NGS re-sequencing throughput capabilities
• This NGS Sample Prep system significantly reduces PCR amplification and normalization complexity to allow for enrichment of regions up to 1MB in a 96 well plate format
• Amplify and equalize with the ease of SequalPrep™ for consistently high coverage of multiple regions of interest

Specifications

General

Exonuclease Activity	3' - 5', 5' - 3'
Fidelity (vs. Taq)	4 X
Hot Start	Built-In Hot Start
Overhang	Mixed
Polymerase	SequalPrep Long Polymerase
Product Line	SequalPrep
Quantity	1,000 units
Reaction Format	Separate Components
Shipping Condition	Dry Ice
Size (Final Product)	20 kb or less
GC-Rich PCR Performance	Medium
Reaction Speed	Standard
Unit Size	Each

FAQs

Question: What quantity does A10498 provide?

Answer:This package contains 1,000 units of the SequalPrep Long PCR Kit with dNTPs.

Question: What type of PCR does it support?

Answer:It supports long-range genomic DNA amplification, including suitable targets up to about 20 kb.

Question: Does the product include a PCR instrument?

Answer:No. It is a long-PCR reagent kit, not a thermal cycler.

Application Note

Applied Biosystems SequalPrep Long PCR Kit with dNTPs is an amplification system for long genomic DNA targets used in molecular biology and sequencing preparation. The kit combines SequalPrep long-range polymerase chemistry with dNTPs for generating extended PCR amplicons from suitable DNA templates. It supports long-range amplification up to approximately 20 kb in appropriate sample and primer conditions, facilitating targeted enrichment, resequencing, genetic analysis, and characterization of large genomic regions. The 1,000-unit package provides polymerase capacity for multiple reactions and is intended for research workflows requiring long DNA products. Long-amplicon success depends on primer design, template integrity, target composition, and cycling conditions. The product is a reagent kit rather than a thermal cycler, sequencing instrument, or DNA purification cartridge. It is suited to laboratories preparing extended PCR targets for downstream sequencing.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.