Quantity: 1 kit
Unit Size: Each
The PichiaPink™ Secreted Protein Expression Kit is a new recombinant protein expression system based on the yeast Pichia pastoris. The PichiaPink™ Secreted Protein Expression Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein. The PichiaPink™ Secreted Protein Expression Kit comes with our new Pichia strains, the pPINK-HC and pPINK-LC vectors, and secretion signal sequences for production of your protein.
• Get started with secreted protein production inPichia pastoris.
• Screen yourPichiatransformants rapidly by auxotrophy and color selection.
• Reduce protein degradation with protease-deficientPichia pastorisstrains.The PichiaPink™ Secreted Protein Expression Kit comes with:
• PichiaPink™ Secreted Protein Vector Kit (Cat. No. A11153)
• PichiaPink™ Expression Strain Set (Cat. No. A11154)
• PichiaPink™ Media Kit (Cat. No. A11156)A New Way to Screen Pichia TransformantsThe PichiaPink™ Secreted Protein Expression Kit uses adenine auxotrophy to select forPichiatransformants. The PichiaPink™ strains have theade2genotype and require complementation with theADE2gene to grow without adenine. Use of an auxotrophic marker also makes it easier to maintain transformants. No antibiotics are required to maintain your transformants.In addition, you can screen forPichiatransformants by color. The untransformed PichiaPink™ strains appear as pink colonies while transformed PichiaPink™ strains appear as white colonies (Fig 1).Secrete Your Proteins with the pPINKα-HC VectorThe PichiaPink™ Secreted Protein Expression Kit comes with the pPINKα-HC vector. The pPINKα-HC vector has the α-mating factor signal sequence built-in and allows your protein to be secreted into the media. Secreting your protein into the media makes harvesting and purifying your protein easier. The pPINKα-HC vector is built around theADE2gene for complementing theade2-deficientPichiastrains. This vector uses the methanol-inducedAOX1promoter to express your protein. Further, the pPINKα-HC vector is designed to be integrated at high-copy-number in yourPichiatransformants.The PichiaPink™ Secreted Protein Vector Kit (Cat. No. A11153) can be ordered separately to refill the PichiaPink™ Secreted Protein Expression Kit.Keep Your Proteins IntactThe PichiaPink™ Secreted Protein Expression Kit comes with the PichiaPink™ Expression Strain Set. This set contains the 4Pichia pastorisstrains for use with the PichiaPink™ Yeast Expression System. All 4 strains have theade2genotype. In addition, 3 strains include knockouts in 2 protease genes. Protease-deficient strains reduce the need for protease inhibitors when growing your cells and improve the yield of your protein. Test a single protease knockout or the double protease knockout to find the one that works best for you.You can order the PichiaPink™ Expression Strain Set (Cat. No. A11154) separately to refill the PichiaPink™ Secreted Protein Expression Kit .Media Pouches to Get You StartedThe PichiaPink™ Media Kit is a set of convenient prepackaged media pouches for use with the PichiaPink™ Secreted Protein Expression Kit. The PichiaPink™ Media Kit contains media needed to grow PichiaPink™ strains from frozen stocks to cultures ready for transformation and selection. You can order the PichiaPink™ Media Kit (Cat. No. A11156) separately to refill the PichiaPink™ Secreted Protein Expression Kit.Why Choose the PichiaPink™ Yeast Expression System?The PichiaPink™ Yeast Expression System is based on the yeastPichia pastoris. Advantages ofPichia pastorisinclude rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years,Pichia pastorishas been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire
[email protected] Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.Related Links
• Learn more about the PichiaPink™ Yeast Expression System.
• Learn more about our other protein expression systems.References1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]
