Thermo Fisher, A1122205, CD OptiCHO™ AGT™ Medium, 10 L

Name: CD OptiCHO™ AGT™ Medium, 10 L
Brand: Thermo Fisher
SKU: A1122205

Quantity: 10 L, Unit Size: Each, CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco™ CD CHO's legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Than

Catalog Number: A1122205

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Category

Chemically Defined Media

Pack Size10 L

TagsCell Culture Media Supplements and Reagents Cell Target Type Cell Culture Media Chinese Hamster Ovary Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 L
Unit Size: Each
When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is 'finicky,' i.e. hard to grow or adaptWhen to Consider Another Gibco™ MediumConsider CD FortiCHO™ medium (Cat. No. A1148301) when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processingConsider CD CHO medium (Cat. No. 10743029) when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)Consider CD DG44 medium (Cat. No. 12610010) when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)Liquid Media FormulationsGibco™ AGT™ media are also available in a liquid format (Cat. No. 12681011) that offers the same performance and formulation as the AGT™ media format. Gibco™ liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.Design Your Own Media Specific to Your NeedsWhether it's a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Specifications

General

Cell Line	CHO
Culture Environment	CO2
Endotoxin Level	Low
Format	Securitainer Capped Vial
Included Antibiotics	No antibiotics
Inorganic Salts	Magnesium, Sodium Phosphate
Product Line	AGT, Gibco
Product Type	CD OptiCHO AGT Medium
Purity or Quality Grade	Certified
Quantity	10 L
Reconstituted Volume	10 L
Remarks	Medium does not support wild type DG44 cells
Shipping Condition	Wet Ice
Classification	Chemically-defined
Culture Type	Suspension Cell Culture
Form	Powder (AGT)
Serum Level	Serum-free
Sterility	Non-sterile
With Additives	Sodium Bicarbonate
Without Additives	No Phenol Red
Unit Size	Each

FAQs

Question: What volume does this AGT format prepare?

Answer:The container reconstitutes to 10 L of medium.

Question: Is the formulation chemically defined?

Answer:Yes. It is chemically defined, serum-free, protein-free, and animal-origin free.

Question: Does the formulation contain glutamine?

Answer:No. Glutamine is not included in this formulation.

Application Note

CD OptiCHO™ AGT™ Medium is a chemically defined, protein-free, serum-free, animal-origin-free medium for suspension culture of recombinant CHO cells. The AGT granular dry format is designed to reconstitute to 10 L and supports fed-batch process development and production workflows. The formulation contains high glucose, sodium pyruvate, and sodium bicarbonate, while glutamine and phenol red are not included. Its low-osmolality formulation is intended for CHO processes in which feeding strategy is optimized to improve culture performance and recombinant-protein productivity. The medium is particularly relevant for transfected CHO lines outside the CHO K1, GS CHO, or CHO-S groups and for lines that are difficult to adapt or grow. Store the dry medium at 2–8 °C in dark, dry conditions.

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