Thermo Fisher, A1148301, CD FortiCHO™ Medium

Name: CD FortiCHO™ Medium
Brand: Thermo Fisher
SKU: A1148301

Quantity: 1 L, Unit Size: Each

Catalog Number: A1148301

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Category

TagsCell Culture Media Supplements and Reagents Cell Target Type Cell Culture Media Chinese Hamster Ovary Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 L
Unit Size: Each
CD FortiCHO™ medium is specifically designed to offer the highest batch and fed-batch culture performance and yield with recombinant CHO cells in a chemically defined environment. The protein-free, animal origin component-free formulation provides consistency and helps reduce the need to screen for adventitious agents. With CD FortiCHO™ medium cell cultures last 2+ days longer than competitive alternatives, which means decreased HCP (Host Cell Protein) concentration for removal in downstream purification. CD FortiCHO™ medium is fortified to support maximal recombinant CHO cell growth and protein expression in suspension batch culture, offering 120% greater growth and production than select competitive media with simple glucose supplementation. CD FortiCHO™ medium can provide up to 75% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with CD EfficientFeed™ C nutrient supplement. When to Use CD FortiCHO™ Medium
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processingWhen to Consider Another Gibco™ MediumConsiderCD OptiCHO™ mediumwhen:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is finicky, i.e. hard to grow or adaptConsiderCD CHO mediumwhen:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)ConsiderCD DG44when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)Dry Media FormulationsFor early access to CD FortiCHO™ AGT™ medium, please contactGibco™ Technical Services.Design Your Own Media Specific to Your NeedsWhether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. WithGibco™ cGMP Media Custom Servicesyou can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Specifications

General

Cell Line	CHO
Concentration	1 X
Endotoxin Level	Low
Format	Bottle
Included Antibiotics	No antibiotics
Product Line	Gibco
Product Type	CD OptiCHO AGT Medium
Quantity	1 L
Shipping Condition	Room Temperature
Classification	Chemically-defined
Culture Type	Suspension Cell Culture
Form	Liquid
With Additives	Sodium Pyruvate
Without Additives	No Glutamine
Unit Size	Each

FAQs

Question: What cells is CD FortiCHO Medium designed for?

Answer:It supports suspension culture of recombinant CHO cells in batch and fed-batch workflows.

Question: Does the formulation contain glutamine or antibiotics?

Answer:No. It contains neither glutamine nor antibiotics.

Question: How is the 1 L medium supplied and stored?

Answer:It is a liquid medium supplied as 1 L and stored at 2–8°C protected from light.

Application Note

CD FortiCHO™ Medium is designed as a chemically defined, serum-free basal production medium for suspension cultures of recombinant Chinese hamster ovary cells. The 1 L liquid medium supports batch and fed-batch bioprocess development in which CHO growth and recombinant protein expression must be maintained in a controlled nutritional environment. Its formulation is protein-free and free of components of animal origin, avoiding proteins and undefined serum components in the basal medium. It includes sodium bicarbonate and sodium pyruvate but does not contain glutamine, phenol red or antibiotics, allowing process developers to control the addition of certain components in their culture strategy. The formulation is designed to support high viable-cell growth and recombinant production in CHO suspension cultures, including workflows that incorporate compatible nutritional feeds after initial growth. The supplied product is one liter of ready-to-use liquid medium, not an AGT dry-powder preparation. Its consistent chemically defined composition supports comparative evaluation of culture conditions and downstream impurity profiles. Store in the dark at 2–8°C; the stated stability under recommended storage is 12 months.

Ordering

Notice

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