Thermo Fisher, A11667, MembranePro™ Functional Protein Expression Kit

Name: MembranePro™ Functional Protein Expression Kit
Brand: Thermo Fisher
SKU: A11667

Quantity: 10 reactions kit, Unit Size: Each

Catalog Number: A11667

Pricing

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Category

Mammalian Expression Systems

Pack Size10 reactions

TagsProtein Purification Protein Analysis Reagents In vitro Protein Expression Systems

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 reactions kit
Unit Size: Each
For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably. Benefits of MembranePro™ include:
• Proteins are displayed on human (or other mammalian) cell membranes
• Cellular quality control and mammalian posttranslational processing helps produce functional protein
• Proteins bud off as lipoparticles and are easily collected from the culture medium
• Lipoparticles are enriched with expressed receptorsMembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:1. Clone your gene of interest using a TOPO™ vector2. Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells3. Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix4. Store lipoparticles at -80 C or proceed to downstream assaysMembranePro™ products are offered in two configurations – as an expression kit (TOPO™ vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.

Specifications

General

Cell Line	293FT
Delivery Type	Virus-Like Particles (VLPs)
Expressed Protein Type	Membrane Protein
Expression Mechanism	Cell-Based Expression
For Use With (Application)	Membrane Protein Expression
High-throughput Compatibility	High-throughput Compatible
Product Line	MembranePro
Quantity	10 reactions kit
Selection Agent (Eukaryotic)	Blasticidin
Selection Marker Eukaryotic	BsdR
Expression System	Mammalian
Promoter	EF-1α
Type	MembranePro Functional Protein Expression Kit
Unit Size	Each

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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Thermo Fisher, A11667, MembranePro™ Functional Protein Expression Kit

Send an RFQ.

General

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