Description
Description: Qualyfast Legionella PCR Assay
No. of Reactions: 8 Reactions
No. of Tests: 48 Tests
Unit Size: Each
Minimizes false negatives
• Free DNA Inactivator removes DNA from non-viable sources commonly found in environmental water samples.
• DNA Extraction Kits remove environmental contaminants minimizing potential inhibition effects.
• PCR Assays with simultaneous co-amplification of an Internal Application Control allowing determination of potential inhibition effects.Increases accuracy
• Quantification Standards enable PCR detection ofLegionellaspecies to be quantified within the 25-25,000 Genomic Unit range.
• Increases throughput: Multiplexed PCR of the characteristic DNA sequences for species within the Legionella genus and the specific species L. pneumophilia.Easy-to-use
• PCR Assay kits contain strips with eight reaction tubes containing lyophilized PCR reagents, DNA free solution and pre-dosed positive-control tubes.
• Quantification Standards are pre-aliquoted and lyophilized in individual tubes.
• DNA Extraction Kits contain all the consumables required to extract DNA from environmental water samples.Optimized workflow
• Complete end-to-end workflow covering sample preparation using DNA Extraction Kits and Free DNA Inactivator, detection using multiplex Legionella PCR Assay and evaluation of results using Quantification Standards
• ISO compliant: workflow is compliant with the protocol, experimental design and calculation methods described by ISO/TS 12869:2012The qualyfast™ Legionella PCR Assay contains:
• Pre-dosed and lyophilized PCR reagents in strips of 8 reaction tubes
• A strip of 8 reaction tubes containing positive controls
• DNA-free solutionThe qualyfast™ DNA Extraction Kit I is designed for use with clear water samples, as acquired from water distribution systems, drinking water and other sources that contain minimal suspended particles. The kit contains:
• Single-use, sterile membrane filters
• Lysis buffer for enhanced isolation of microorganisms from filter membranes
• Lysis enzyme for DNA extractionThe qualyfast™ DNA Extraction Kit II is designed for use with dirty water, biofilm and sediment samples. The kit contains:
• Single-use, sterile membrane filters
• Pre-lysis buffer
• Lysis solution & bead beating lysis tubes
• Spin filter
• Post-lysis filtration columns and reagents
• All collection tubes required for sample handling during extractionThe qualyfast™ Free DNA Inactivator Kit contains:
• DNA inactivation enzyme (lyophilized)
• DNA inactivation buffersThe qualyfast™ Legionella Quantification Standards Kit contains:
• Four different quantified standards, each packaged in strips of 8 reaction tubes, enabling 8 analysis sessions
• DNA-free solution for reconstitution of lyophilized reagents and negative-controlThe qualyfast™ Legionella workflow is compatible with the Applied Biosystems™ 7500 Fast Food Safety Real-Time PCR System or Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System.
