Thermo Fisher, A24229, MAX Efficiency™ Transformation Reagent for Algae

Name: MAX Efficiency™ Transformation Reagent for Algae
Brand: Thermo Fisher
SKU: A24229

Quantity: 40 reactions, Unit Size: Each

Catalog Number: A24229

Pricing

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Category

Sequencing Reaction Kits

Pack Size40 reactions

TagsBiochemical Reagents Nucleic Acid Introduction Reagents Electroporation Transformation Kits

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 40 reactions
Unit Size: Each
MAX Efficiency™ Transformation Reagent for Algae is a buffer that enhances transformation efficiency for multiple strains of Chlamydomonas species. One of the biggest hurdles in research and development with Chlamydomonas has been the introduction of exogenous DNA into Chlamydomonas strains due to rigid cell walls. Methods such as glass bead agitation, electroporation, and microparticle bombardment are available but often result in low transformation efficiency.MAX Efficiency™ Transformation Reagent for Algae increases the permeability of the Chlamydomonas cell wall and facilitates increased delivery of DNA into the cell nucleus by electroporation. Simply grow the Chlamydomonas cells to early log phase, harvest by centrifugation, and wash twice with MAX Efficiency™ Transformation Reagent prior to electroporation. To date, we have seen >200-fold increase in transformation efficiency over previously recommended conditions in 10 different Chlamydomonas strains, including wild type and mutants, using circular or linear DNA, as well as PCR fragments.
• Obtain >1000 transformants/μg DNA for cell wall(+) strains
• Obtain >100 transformants/μg DNA for cell wall(-) strains
• Transform circular or linear DNA or PCR fragmentsFor Research Use Only. Not for use in diagnostic procedures.

Specifications

General

Cell Line	Chlamydomonas
Expression System	Algae
For Use With (Application)	Gel Electrophoresis
High-throughput Compatibility	Not High-throughput Compatible (Manual)
No. of Reactions	40 Reactions
Product Line	GeneArt
Product Type	Transformation Reagent
Quantity	40 reactions
Selection Agent (Eukaryotic)	Hygromycin
Unit Size	Each

Ordering

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Thermo Fisher, A24229, MAX Efficiency™ Transformation Reagent for Algae

Send an RFQ.

General

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