| For Use With (Application) | RNA Sequencing |
|---|---|
| For Use With (Equipment) | Ion Proton™ System |
| Libraries | Targeted Sequencing Library |
| Product Line | AmpliSeq |
| Product Type | Transcriptome Human Gene Expression Kit |
| Quantity | 96 Assays |
| Sample Source | Total RNA |
| Workflow Step | Library Generation |
| Sequencing Type | Transcriptome Sequencing |
| Unit Size | Each |
Answer:This configuration supports 96 assays.
Answer:The panel measures expression of more than 20,000 human RefSeq genes.
Answer:The workflow can start with approximately 10 ng of total RNA.
Ion AmpliSeq™ Transcriptome Human Gene Expression Kit enables targeted RNA sequencing of more than 20,000 human RefSeq genes for broad gene-expression profiling. This configuration supports 96 assays. The workflow can start with as little as 10 ng of total RNA, including RNA from challenging or degraded samples such as FFPE tissue. Targeted amplification generates libraries suitable for Ion semiconductor sequencing and provides a wider quantitative dynamic range than conventional microarray-based expression profiling. The kit includes key library-generation reagents such as 5X Ion AmpliSeq HiFi Mix, FuPa Reagent, and Switch Solution. Ion Xpress barcode adapters can be incorporated for multiplexed sequencing. Generated libraries are suitable for approximately 200-base sequencing reads.
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