| Calibrated Dye | FAM, ROX, SYBR™, VIC |
|---|---|
| For Use With (Application) | Spectral Calibration |
| For Use With (Equipment) | QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Pro, QuantStudio™ 7 Pro, QuantStudio™ 6 Flex (Software version 1.6 or later), QuantStudio™ 7 Flex (Software version 1.6 or later) |
| Format | 96-well, 0.2 mL |
| PCR Method | qPCR |
| Plate Compatibility | 96-well, 0.2 mL |
| Quantity | 1 plate |
| Shipping Condition | Dry Ice |
| Volume (Metric) Thermal Block Sample | 0.2 mL |
| Product Type | Calibration Plate |
| Unit Size | Each |
Answer:The plate contains FAM, VIC, ROX, and SYBR dye formulations.
Answer:It is a preloaded 96-well plate for 0.2 mL real-time PCR blocks.
Answer:It establishes pure dye spectra and multicomponent values so the instrument can distinguish overlapping fluorescence signals.
QuantStudio™ 3/5 Spectral Calibration Plate 1 is used to establish dye-specific fluorescence spectra on compatible real-time PCR instruments. The preloaded 96-well, 0.2 mL plate contains FAM™, VIC™, ROX™, and SYBR™ dye formulations. During spectral calibration, the instrument measures the characteristic signal from each dye and creates multicomponent values that allow overlapping fluorescence to be separated during later qPCR runs. Accurate spectral calibration is especially important for multiplex assays in which several reporter or reference dyes are measured in the same reaction plate. This plate is designed for QuantStudio 3 and 5 systems and is also supported in specified calibration workflows for newer QuantStudio Pro and Flex instruments. It is supplied ready for calibration use and should be stored frozen at approximately –20°C, protected from unnecessary warming before the procedure.
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