Quantity: 1 x 10 mL
Unit Size: Each
Request an evaluation sampleThis single-tube, 4X format facilitates easy reaction set up—just add user-supplied assay and sample (Figure 1). With reproducible performance even in the presence of inhibitors, coupled with stringent quality control to minimize lot-to-lot variation, TaqPath 1-Step Multiplex Master Mix (No ROX) is a superior choice for your multiplex diagnostic testing or development needs.Features of the TaqPath 1-Step Multiplex Master Mix include:
• High sensitivity—to detect low-copy targets with reproducible CT results
• Six logs of dynamic range—with both RNA and DNA targets
• Optimized for multiplexing—up to four targets simultaneously without a passive reference dye
• Tolerant of inhibitors—commonly found in clinical and biopharma samples
• Advanced manufacturing—production and process controls ensure lot-to-lot consistency
• Compliance Package—containing most frequently requested compliance documents, available below, commonly used to comply with internal quality systems and regulatory requirements in molecular diagnostic assay and drug developmentHigh sensitivityTo enable reproducible detection of low-titer pathogen and transcripts, TaqPath 1-Step Multiplex Master Mix (No ROX) has been optimized as a higher-concentration 4X master mix that allows you to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Figure 1 shows the consistency of results obtained when detecting 10 copy inputs of RNA target.Wide dynamic range compatible with RNA and DNATaqPath 1-Step Multiplex Master Mix (No ROX) has been optimized to provide high specificity and dynamic range for use with both RNA and DNA targets. Since virology labs often test for both RNA and DNA viruses, TaqPath 1-Step Multiplex Master Mix (No ROX) is designed to use a single protocol to assay both types of nucleic acid. This input flexibility can help streamline the number of different workflows in your lab to improve efficiency. Figure 2 demonstrates the excellent PCR linearity across a 6-log RNA dilution series, while Figure 3 exhibits a 6-log linear dynamic range for both RNA and DNA targets.Optimized for multiplexingTaqPath 1-Step Multiplex Master Mix has been validated for multiplexing up to four targets simultaneously, allowing for additional targets and/or controls to be run simultaneously for efficiency or quality control purposes. The mix does not include a passive reference dye, enabling the measurement of the JUN dye, or a similar emission wavelength dye, in the channel previously used to measure ROX dye. Figure 3 demonstrates TaqPath 1-Step Multiplex Master Mix (No ROX) performance in 4-plex reactions with both RNA and cDNA targets.Inhibitor tolerantUnlike other master mixes on the market, the unique proprietary formulation of TaqPath 1-Step RT-qPCR Master Mix (No ROX) allows robust performance even in the presence of PCR inhibitors typically found in biopharmaceuticals, molecular diagnostics, and research applications. Optimized performance in the presence of such substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, helps increase confidence when working with a variety of complex clinical or biopharma samples. TaqPath master mixes demonstrate increased tolerance of inhibitors compared to competitor mixes (Figure 4).Manufacturing production and process controlsTaqPath 1-Step Multiplex Master Mix (No ROX) is manufactured under a strong ISO13485 quality management system that uses traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath product manufacturing is designed to produce consistent product performance lot after lot. Figure 5 demonstrates CT lot-to-lot consistency is preserved across multiple assays with different attributes and input target levels. The stringent QC is important for a variety of applications, making the master mix ideal for bioprocess quality control, biologics adventitious testing, molecular diagnostics, and research applications.General purpose reagent manufactured under cGMPTaqPath 1-Step RT-qPCR Master Mix is a General Purpose Reagent (GPR), labeled For Laboratory Use and manufactured in an ISO 13485-certified and FDA-registered facility that adheres to cGMP principles.Compliance PackageIn an effort to efficiently support your compliance needs, frequently requested compliance documents are available here:
• Statement of Quality relating to the current good manufacturing practices (cGMP) to which TaqPath master mixes adhere
• Detailed lot-specific Certificate of Analysis (COA) (see below in Documents & Downloads section)
• Product Stability Statement
• Supplier Evaluation SupportCompleted Self AssessmentISO certificates by manufacturing site
• For EU Reach compliance-related inquiries, email
[email protected]• BSE/TSE Statement
• Change Control and Notification RegistrationNot finding the compliance document you're looking for? Technical Support is here to help.Related productsTaqPath 1-Step Master Mix is available in three different formulations:TaqPath 1-Step qRT-PCR Master Mix (Cat. No. A15300)—includes ROX dye as the passive referenceTaqPath 1-Step Multiplex Master Mix (Cat. No. A28525)—includes MUSTANG PURPLE dye as the passive referenceTaqPath 1-Step Multiplex Master Mix (no ROX) (this page)—does not include a passive reference dye
