Thermo Fisher, A29377, Precision gRNA Synthesis Kit

Quantity: 25 reactions
Unit Size: Each
Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. GeneArt Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Starting with two short single-stranded oligos that code for the target sequence, the gRNA template is assembled with a T7 promoter in a short one-pot PCR reaction. The assembled product is then used as template in an in vitro transcription (IVT) reaction followed by a rapid purification step, yielding transfection-ready gRNA in as little as four hours. Resulting gRNA can also be co-transfected with our ready-to-transfect GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches.Features of the GeneArt Precision gRNA Synthesis Kit include:
• Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly
• High yield (>10 ug) and concentration (>200 ng/uL) of gRNAFor research use only. Not for use in diagnostic procedures.