Quantity: 1 x 10 mL
Unit Size: Each
Designed for multiplex SNP genotypingA TaqMan™ SNP Genotyping Assay targets a single SNP locus (a specific genomic position) and includes two PCR primers and two allele-specific probes. Each probe hybridizes to the same genomic region but differs by one nucleotide, allowing detection of one of the two possible alleles at that locus. This design enables allelic discrimination within a single qPCR reaction.TaqPath™ ProAmp™ Master Mix supports singleplex and multiplex SNP genotyping, including 2-SNP multiplex configurations (two SNP loci per reaction; four allele-specific probes total). This multiplexing approach increases data output per sample while maintaining confident allelic discrimination and assay performance.Six SNP loci per reaction multiplexing requires a custom TaqPath ProAmp formulation without ROX.Benefits of TaqPath™ ProAmp™ Master Mix
• Exceptional data quality—high specificity, reproducibility, and dynamic range* forSNP genotyping and CNV analysis
• Inhibitor tolerance—robust performance with samples derived fromblood, buccal swabs, and card punches
• Optimized for SNP genotyping from crude lysates—supports accurate SNP genotyping without DNA purification
• 72-hour pre-PCR benchtop stability with purified DNA—ideal for automated and high-throughput workflows, enabled by the proprietaryDual-Lock™ Taq DNA hot-start mechanism
• Multiplex flexibility—supportssingleplex through four-target SNP detection per reaction
• Manufacturing consistency—produced in anISO 13485-certified facility
• Compliance Package available—includes commonly requested quality and regulatory documentationAccurate SNP genotyping from purified DNA or crude lysatesTaqPath™ ProAmp™ Master Mix delivers accurate and reproducible SNP genotyping results from either purified genomic DNA or crude lysate inputs. When combined with the DNA Extract All™ Reagents Kit, the master mix supports robust singleplex and 2-locus multiplex SNP genotyping directly from crude lysates prepared from blood and buccal swabs.Internal testing demonstrated 100% concordance between SNP genotype calls obtained from crude lysates and purified genomic DNA using selected 2-SNP multiplex assay configurations, confirming reliable allelic discrimination without DNA purification.Accurate SNP genotyping from crude lysates with improved performance versus alternative master mixesIn internal evaluations using TaqMan™ SNP Genotyping Assays and crude blood lysates, TaqPath™ ProAmp™ Master Mix demonstrated improved SNP genotype call accuracy and clearer allelic discrimination compared to selected alternative qPCR master mixes. The formulation produced tight genotype cluster separation and consistent SNP locus calls across replicates, supporting confident genotyping performance even in the presence of inhibitors commonly found in biological samples.High-accuracy copy number variation (CNV) analysisIn addition to SNP genotyping, TaqPath™ ProAmp™ Master Mix supports accurate copy number variation (CNV) analysis when used with purified genomic DNA. Crude lysate sample inputs are not recommended for CNV analysis.The formulation enables reliable copy number determination across multiple gene targets, including CYP2D6 intron 2 and CYP2D6 intron 6, with accurate quantification in samples containing one to four copies. Internal comparisons demonstrated improved copy number precision and reproducibility compared to selected alternative qPCR master mixes when purified genomic DNA was used as the input.Part of a streamlined Sample-to-Genotype workflowTaqPath™ ProAmp™ Master Mix integrates seamlessly into a complete Sample-to-Genotype workflow, including:
• DNA Extract All™ Reagents Kitfor rapid preparation of crude lysates forSNP genotyping
• TaqMan™ Predesigned SNP Genotyping AssaysorTaqMan™ DME Genotyping Assays
• Purified genomic DNAforcopy number variation analysis
• QuantStudio™ Real-Time PCR Instruments
• Diomni™ Design and Analysis Softwarefor multiplex genotyping data analysisThis workflow enables faster turnaround and reduced hands-on time for SNP genotyping, while supporting accurate CNV analysis using purified DNA when required.General purpose reagents manufactured under cGMPTaqPath™ master mixes are general purpose reagents labeled For Laboratory Use and are manufactured in an ISO 13485-certified and FDA-registered facility that adheres to current Good Manufacturing Practice (cGMP) principles.Compliance PackageFrequently requested compliance documents are available, including:
• Statement of Quality describing adherence to cGMP
• Lot-specificCertificate of Analysis (COA)
• Product Stability Statement
• Supplier Evaluation Support documentation
• Completed Self Assessment
• ISO certificates by manufacturing site
• BSE/TSE Statement
• Change Control and Notification RegistrationFor EU REACH compliance-related inquiries, email
[email protected] finding the document you need? Technical Support is available to help.* Dynamic range depends on assay design, template concentration, and master mix formulation; individual results may vary.
