Thermo Fisher, A31746, TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 0.2 mL

Name: TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 0.2 mL
Brand: Thermo Fisher
SKU: A31746

Quantity: 0.2 mL, Unit Size: Each

Catalog Number: A31746

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Category

One-Step RT-PCR Kits

Pack Size0.2 mL

TagsPCR Equipment and Supplies PCR Supplies Real Time PCR Products Real Time PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 0.2 mL
Unit Size: Each
The TaqMan Arbovirus Triplex Kit enables the simultaneous detection and differentiation of RNA from dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) in urine and serum. It is a lyophilized, multiplex, one-step real-time PCR Applied Biosystems TaqMan assay for the molecular detection of dengue, chikungunya, and Zika RNA. The kit is optimized for use with the Applied Biosystems QuantStudio 5 Real-Time PCR System, but is also suitable for the 7500 and other QuantStudio systems. It includes a primer-probe set for human PPIA (cyclophilin A) as an endogenous extraction, RT-PCR and PCR amplification control. The kit is compatible with RNA extracted from human urine or serum using the MagMAX Pathogen RNA/DNA Kit. The Zika virus sequence used to develop these primers and probes is from a 2014 research paper by Pyke et al (1).Features of the kit:• Lyophilized with one-step RT-PCR master mix; simply add RNA• Simple workflow for detection of Zika, Dengue, and Chikungunya virus RNA from urine or serum• Easy to use and environmentally friendly. No cold chain management required; shipped and stored at room temperature. Contents:• Twelve 8-well strips tubes for 96 total reactions per kit• 0.2-mL tube format, compatible with 96-well block• Primer-probe detection with 4 probe reporter dyes: --FAM-Zika --VIC-Dengue --ABY-Chikungunya --JUN-PPIA cyclophilin1. Pyke AT, Daly MT, Cameron JN, Moore PR, Taylor CT, Hewitson GR, Humphreys JL, Gair R. Imported zika virus infection from the cook islands into australia, 2014. PLoS Curr. 2014 Jun 2;6.

Specifications

General

Description	Multiplex standard strip (0.2mL, 12 x 8-well strip tubes)
For Use With (Equipment)	Any qPCR insturment
Product Line	TaqMan
Quantity	0.2 mL
Detection Method	Primer-probe
For Use With (Application)	Pathogen Detection
Unit Size	Each

FAQs

Question: Does 0.2 mL indicate reagent volume?

Answer:No. It identifies the reaction-tube and PCR-block format. The kit supplies 96 reactions in twelve eight-tube strips.

Question: Which viral targets are detected?

Answer:Zika virus, dengue virus serotypes 1–4 and chikungunya virus RNA, together with human PPIA as an endogenous process control.

Question: What type of specimen and PCR workflow are suitable?

Answer:The kit uses extracted RNA from human serum or urine research samples in a multiplex one-step real-time RT-PCR workflow on compatible instruments.

Application Note

The Applied Biosystems TaqMan Arbovirus Triplex Kit is a lyophilized, multiplex one-step real-time RT-PCR assay for research detection and differentiation of Zika virus, dengue virus and chikungunya virus RNA. The 96-reaction kit consists of twelve strips of eight reaction tubes designed for a 0.2 mL real-time PCR block; 0.2 mL describes the tube format, not the liquid reagent fill volume. Fluorescent detection channels distinguish Zika (FAM), dengue (VIC) and chikungunya (ABY), while human PPIA (JUN) provides an endogenous process control. The assay supports extracted RNA from human serum or urine research specimens and includes the lyophilized one-step RT-PCR chemistry. Sample extraction, instrument setup, controls and interpretation must follow the assay workflow. It is not a standalone clinical diagnostic determination.

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