Description
Quantity: 2 Plates
Unit Size: Each
Features of the TaqMan Advanced Serum/Plasma 96-Well Plates include:
• Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
• Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
• Updated content—assay content updated for mirBase v.21
• Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
• Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA familiesProduct details:
• Number of plates:2
• Number of unique miRNAs (not including exogenous controls):188
• Exogenous controls:cel-miR-39-3p, ath-miR159a
• Endogenous control:hsa-miR-16-5p
• Compatible with the following Applied Biosystems qPCR instruments:Viia7, 7500, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
• Recommended master mix:TaqMan Fast Advanced Master MixNotes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
