| Cell Type | Neural Cells |
|---|---|
| Concentration | 50 X |
| Culture Type | Mammalian Cell Culture |
| Product Line | B-27 |
| Quantity | 10 mL |
| Serum Level | Serum-Free |
| Shelf Life | 12 Months |
| Shipping Condition | Dry Ice |
| Tested For | Endotoxin, Performance, Sterility |
| Classification | Serum-free |
| Form | Liquid |
| Product Type | Cell Culture Supplement |
| Sterility | Sterile |
| Unit Size | Each |
Answer:It is optimized for use with Neurobasal Plus Medium.
Answer:The pack contains 10 mL of 50X concentrate, sufficient for up to 500 mL at 1X.
Answer:Store at −5 to −20°C protected from light.
B-27™ Plus Supplement (50X) is designed to promote survival, maturation and sustained culture of neuronal cells when used with compatible neuronal basal media. This next-generation serum-free B-27 formulation is optimized for use alongside Neurobasal Plus Medium and supports cultures of primary rodent neurons and human stem-cell-derived neurons. It enables experimental systems requiring viable neuronal populations over extended culture periods, including studies of neuronal morphology, maturation and function. The liquid supplement is supplied as 10 mL of 50X concentrate, providing enough material for up to 500 mL of medium when used at the standard 1X working concentration. Its formulation, production process and quality-control requirements differ from the original B-27 and minus-vitamin-A variants; it should not be described as the vitamin-A-free supplement used specifically to maintain undifferentiated neural precursors. The product is serum-free and sterile, with testing for endotoxin, sterility and performance. It is shipped frozen and should be stored at −5 to −20°C protected from light. It is a concentrated supplement rather than a complete basal medium.
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