Thermo Fisher, A40007051, MultiShot™ BL21(DE3) Chemically Competent <i>E. coli</i> Cells, 96-well plate

Name: MultiShot™ BL21(DE3) Chemically Competent E. coli Cells, 96-well plate
Brand: Thermo Fisher
SKU: A40007051

Quantity: 1 x 96 Well Plate (20 μL/Well), Unit Size: Each, Invitrogen MultiShot BL21(DE3) Chemically Competent E. coli Cells are suitable for the expression of non-toxic heterologous genes. They are pre-aliquoted into a 96-well PCR plate to increase transformation productivity.

Catalog Number: A40007051

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Competent Cells

TagsCompetent Cells Protein Analysis Reagents

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 x 96 Well Plate (20 μL/Well)
Unit Size: Each
Features of MultiShot BL21(DE3) competent cells include:
• High transformation efficiency: >1x10^7cfu/μg pUC19 DNA
• Specifically constructed for high-level expression of recombinant proteins
• Microtiter plate format design convenient for automated high-throughput cloning
• Competent cells pre-aliquoted into 96-well plate, ready for transformation
• S.O.C. Outgrowth medium includedMultiShot BL21(DE3) competent cells in a 96-well plate are designed to facilitate high-throughput transformation compatible with liquid handling equipment. The BL21 (DE3) strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG. BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells.GenotypeF– ompT hsdS_B (r_B–, m_B–) gal dcm (DE3)

Specifications

General

For Use With (Application)	Protein Expression
Includes	pUC19 DNA, S.O.C media
Product Line	MultiShot
Product Type	Chemically competent cells
Quantity	1 x 96 Well Plate (20 μL/Well)
Shipping Condition	Dry Ice
Transformation Efficiency Level	≥1.0 x 10<sup>7</sup>
Format	96-well Plate
Species	E. coli
Unit Size	Each

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Thermo Fisher, A40007051, MultiShot™ BL21(DE3) Chemically Competent <i>E. coli</i> Cells, 96-well plate

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