Thermo Fisher, A40007058, MultiShot™ TOP10 Chemically Competent <i>E. coli</i> Cells, 96-well plate

Name: MultiShot™ TOP10 Chemically Competent E. coli Cells, 96-well plate
Brand: Thermo Fisher
SKU: A40007058

Quantity: 1 x 96 Well Plate (20 μL/Well), Unit Size: Each, Invitrogen MultiShot TOP10 Chemically Competent E. coli Cells are derived from DH10B cells and are one of the most popular laboratory strains. Provided in a clear skirted 96-well microtiter plate to simplify high-throughput workflows and provide transformation efficiencies of >1 x 10^8 cfu/μg control plasmid DNA, Multishot TOP10 cells are a versatile strain that can be used in multiple routine cloning applications.

Catalog Number: A40007058

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Competent Cells

TagsCompetent Cells Protein Analysis Reagents

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 x 96 Well Plate (20 μL/Well)
Unit Size: Each
Features of MultiShot TOP10 competent cells include:
• TOP10 strain ideal for high-efficiency cloning and plasmid propagation
• Microtiter plate format design convenient for automated high-throughput cloning
• mcrA mutation that supports efficient transfection of methylated DNA
• lacZΔM15 allele that permits rapid blue/white screening of transfectants
• endA1 mutation that helps reduce endonuclease activity and improve plasmid DNA yields
• recA1 mutation helps reduce unwanted recombinationMultiShot TOP10 competent cells in 96-well plates are designed to facilitate high-throughput transformation. This format allows all steps of the transformation protocol, up to plating, to take place in the same well, saving time and reducing the chance of contamination. After the addition of DNA, MultiShot TOP10 competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler in the same plate.GenotypeF^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

Specifications

General

For Use With (Application)	Cloning
Includes	pUC19 DNA, S.O.C media
Product Line	MultiShot
Product Type	Chemically Competent Cells
Quantity	1 x 96 Well Plate (20 μL/Well)
Shipping Condition	Dry Ice
Transformation Efficiency Level	≥1.0 x 10<sup>8</sup>
Format	96-well Plate
Species	E. coli
Unit Size	Each

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Thermo Fisher, A40007058, MultiShot™ TOP10 Chemically Competent <i>E. coli</i> Cells, 96-well plate

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