Thermo Fisher, A45221, CTS™ TrueCut™ Cas9 Protein, 5 mg

Quantity: 5 mg
Unit Size: Each
Gibco CTS TrueCut Cas9 Protein is our highest performing CRISPR-Cas9 protein, engineered to deliver maximum ribonucleoprotein (RNP)-editing efficiency. This protein is manufactured in compliance with standards for Ancillary Materials for Cell, Gene and Tissue-Based Products and is subjected to stringent quality specifications. It is provided in a high-concentration and large-scale format for process development and clinical research applications. Features of CTS TrueCut Cas9 Protein include: • Consistent high editing efficiency—superior CRISPR-Cas9 editing efficiency in all tested cell lines including primary immune cells (>90% editing in primary T cells) • Higher stringency manufacturing—manufactured to meet the standards for Ancillary Materials for Cell, Gene, and Tissue-Based Products including USP <1043>, Ph.Eur. 5.2.12, and ISO 20399, following the principles of 21 CFR Part 820 in a FDA-registered manufacturing site • Aseptic manufacturing—entire process completed using single-use technology • Extensive safety testing—includes sterility, endotoxin, mycoplasma, residual nucleic acids, and proteins • Sterile filling—finished product is vialed in a Class A/B (Class 100 and ISO 5 equivalents) cleanroom • Traceability documentation—COA, COO, Drug Master Files, and Regulatory Support Files are available CTS TrueCut Cas9 Protein is a Streptococcus pyogenes Cas9 (wt) protein, produced by recombinant DNA technology in E. coli for genome editing with CRISPR technology. Cas9 protein forms a very stable RNP complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of NLS aids delivery to the nucleus, increasing the editing efficiency and on-target cleavage. If you would like to evaluate our TrueCut Cas9 protein or conduct small-scale research experiments, we recommend using our TrueCut Cas9 Protein v2 (Cat. No. A36497).