Description
Description: RapidFinder Halal ID Kit
Unit Size: Each
Support Halal label claims by testing for the presence of pork/wild boar DNA in food and feed samples to verify if any adulteration or cross-contamination has occurred in purchased ingredients or during manufacture.
• Simple: real-time PCR based assay, no electrophoresis needed
• Flexible: pork/wild boar DNA detected in any food or feed sample, both raw and processed
• Specific: detection of highly specific mitochondrial region from gene 16s common to all pork and wild boar with a limit of detection of 0.0005%
• Reliable: Internal Positive Control (IPC) provides process-control check for PCR inhibitors
• Fast: from sample to result in the same dayHow the RapidFinder™ Halal ID Kit worksAnimal DNA detection and identification is accomplished by real-time PCR using two TaqMan™-MGB probes. The target sequence, detected in the FAM dye channel, specifically detects a mitochondrial DNA sequence specific for Sus scrofa (pork/wild boar). Also included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors. By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control represents a 0.1% pork/wild boar DNA, and by comparing sample results to the control results, a threshold of detection is provided. Sample preparationDNA sample preparation for the RapidFinder™ Halal ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample, rather than the smaller sample sizes accommodated by most other DNA isolation procedures, minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.QuantificationIf a positive result is obtained with the RapidFinder™ Halal ID Kit, most labs will go on to quantify the amount of pork/wild boar DNA in the sample. This can be accomplished using the RapidFinder™ Halal ID Kit in conjunction with the RapidFinder™ Quant Multi-Animal Set (Cat. No. A24399). Part of a suite of solutions for meat species detectionThe RapidFinder™ Halal ID Kit is just one of our many kits available for the sensitive detection of various animal species in food and feed samples. Other RapidFinder™ animal species identification kits available include those for the detection of pork, horse, goat, rabbit, water buffalo, duck, beef, horse, sheep, ruminant, chicken, turkey, and poultry. The RapidFinder™ Animal Species Identification kits are made available through a partnership with Health in Code, S.L.
