Description
Quantity: 10 x 1 mL
Reagent Type: LMNG/CHS Solution (10:1)
Unit Size: Each
Features of maltoside detergents• Lipid-like nonionic detergent• Especially useful for isolating and stabilizing hydrophobic membrane proteins• Preserves activity of membrane protein better than most of the detergents• High-purity compounds with low UV absorptivityn-Dodecyl-β-D-Maltoside n-Dodecyl-β-D-Maltoside (DDM) is especially useful for solubilizing membrane proteins to preserve their activity. This water-soluble nonionic detergent is most often used for the isolation of hydrophobic membrane proteins. Multiple studies have shown that n-dodecyl-β-D-maltoside is a gentle detergent that is often able to preserve protein activity better than many commonly used detergents, including NP-40, CHAPS, and octyl-β-glucoside. Properties of DDM • Aggregation number: 98 (average), 70 to 140 range • Micelle molecular weight: 50,000 g • CMC: 0.17 mM (0.009%, w/v) in water ; 0.12 mM (0.006%, w/v) in 0.2 M NaClDDM/CHS Solution DDM/CHS Solution (10:1) is a ready-to-use solution for solubilization of membrane proteins while maintaining structural integrity and activity. The stable pre-formulated solution contains a mixture of dodecyl-β-D-maltoside (DDM) (100 mg/mL) and cholesteryl hemisuccinate (CHS) (10 mg/mL) in deionized water with final concentrations of 10% and 1%, respectively. Typical working concentrations are 1% DDM/0.1% CHS for protein extraction and 0.1% DDM/0.01% CHS for protein purification. The mixture of nonionic detergent, DDM, with dual hydrophobic/hydrophilic properties and anionic detergent, CHS, aid in the stabilization of large unilamellar vesicles and is suitable for protein solubilization and stabilization for single particle cryo-EM analysis.Additional properties of CHS • Molecular formula: C_31H_50O_4 · C_4H_11NO_3 • Molecular weight: 607.9 g/mol • Detergent: ionizable anionic • Function: stabilizes large unilamellar vesiclesLauryl Maltose Neopentyl Glycol Lauryl Maltose Neopentyl Glycol (LMNG) is an amphiphilic detergent ideally suited to improve solubilization of integral membrane proteins while maintaining structural integrity and activity. LMNG has two linked hydrophobic chains of equal length and two hydrophilic maltoside groups to better mimic the integral membrane protein environment. Micelles are known to aid in solubilization of membrane proteins while preserving structure and function, including G-protein coupled receptors (GPCRs). LMNG is suitable for protein solubilization and stabilization for single particle cryo-EM analysis. Properties of LMNG • Chemical name: 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside • Molecular weight: 1005.19 g/mol • CMC: (H_2O) ∼ 0.01 mM, (0.001%) • Purity: ≥98% all anomers (by HPLC analysis)LMNG/CHS Solution LMNG/CHS Solution (10:1) is a ready-to-use formulation for the solubilization of integral membrane proteins while maintaining structural integrity and activity. The stable pre-formulated solution contains a mixture of lauryl maltose neopentyl glycol (LMNG) (5%) and cholesteryl hemisuccinate (CHS) (0.5%) in deionized water. Typical working concentrations for protein extraction are 1% LMNG/0.1% CHS and 0.01% LMNG/0.001% CHS for protein purification and structure determination. LMNG/CHS micelles aid in solubilization of membrane proteins while preserving structure and function, including G-protein coupled receptors (GPCRs), and is suitable for protein solubilization and stabilization for single particle cryo-EM analysis.n-Undecyl-β-D-Maltopyranoside n-Undecyl-β-D-Maltopyranoside (UDM) has an undecyl (C11) alkyl chain attached to the maltose headgroup. The longer alkyl chain length of UDM contributes to its enhanced solubilization efficiency for certain membrane proteins. The increased hydrophobicity of the undecyl chain may facilitate stronger interactions with hydrophobic regions of membrane proteins, leading to improved solubilization and stabilization. The longer undecyl chain length in UDM may also result in a higher CMC compared to maltosides with shorter alkyl chains. This can affect the formation and stability of micelles in solution.Properties of UDM• CMC: (H_2O) ~0.59 mM(1) (0.029%)• Aggregation number: ~71 (100 mM NaCl, 20 mM HEPES pH 7.5)• Purity: ≥99% β+α (by HPLC analysis)n-Nonyl-Beta-Maltoside n-Nonyl-Beta-Maltoside (NM) has a nonyl (C9) alkyl chain attached to the maltose headgroup. This specific chain length provides NM with distinct solubilization and stabilization properties compared to other maltosides with different alkyl chain lengths, such as octyl or decyl maltoside. The specific alkyl chain length of NM influences its micelle properties and contributes to a relatively low CMC. This property allows for the formation of stable micelles at lower concentrations, important for maintaining the stability and integrity of membrane proteins during solubilization and subsequent experiments.Properties of NM• CMC: (H_2O) ~ 6 mM (0.28%)• Aggregation number: ~55 (100 mM NaCl, 20 mM HEPES pH 7.5)• Purity: ≥99% β+α (by HPLC analysis)n-Octyl-β-D-Maltopyranoside n-Octyl-β-D-Maltopyranoside (OM) has an octyl (C8) alkyl chain attached to the maltose headgroup. The specific alkyl chain length of OM sets it apart from other maltosides with different alkyl chain lengths, such as nonyl or decyl maltoside. The octyl chain length in OM contributes to a relatively low CMC, allowing for the formation of stable micelles at lower concentrations. This property is important for maintaining the stability and integrity of membrane proteins during solubilization and subsequent experiments. OM is versatile and finds applications in various areas of membrane protein research, protein crystallization, and biochemical studies. It can be used in a wide range of experimental conditions, buffer systems, and pH ranges. OM offers flexibility and compatibility with different experimental setups.Properties of OM• CMC: (100 mM NaCl, 20 mM HEPES pH 7.5) ~ 19.5 mM(1) (0.89%)• Aggregation number: ~47 (100 mM NaCl, 20 mM HEPES pH 7.5)• Purity: ≥99% β+α (by HPLC analysis)
