Thermo Fisher, A51743, SureQuant™ IP-MS Sample Preparation Kits

Name: SureQuant™ IP-MS Sample Preparation Kits
Brand: Thermo Fisher
SKU: A51743

Description: Protein A/G Magnetic Beads, Unit Size: Each, Thermo Scientific SureQuant IP-MS Sample Preparation kits (Protein A/G or streptavidin) provide an optimized solution for the immunoprecipitation and MS peptide preparation of samples upstream of LC-MS/MS analysis. These kits have been rigorously verified using numerous multiplexed target antigens with varying expression levels. The SureQuant product family enables the use of the immunoprecipitation (IP) sample prep kits and peptide quant

Catalog Number: A51743

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Category

Protein Transfer Buffers

Pack Size1 ML / vial

TagsProtein Analysis Reagents Protein Mass Spectrometry Sample Prep

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Description: Protein A/G Magnetic Beads
Unit Size: Each
• Complete—kits include reagents for successful immunoprecipitation and MS peptide preparation for target proteins for SRM/PRM analysis • Multiplex—IP-MS kits have been designed for multiplex IP using both Protein A/G and Streptavidin magnetic beads • Flexible—universal reagents enable individualized selection of targets for IP to be used alone or combined with SureQuant peptide quantitation panels • Optimized—simplified and condensed procedure to prepare samples for LC-MS analysis • Low background—binding, wash, and elution buffers optimized to remove contaminants and minimize enrichment of background proteins The SureQuant Protein A/G and Streptavidin IP-MS Sample Preparation kits have been designed to support targeted quantitation using SureQuant or SRM/PRM analysis. The kits contain high-quality Pierce Protein A/G or Streptavidin magnetic beads together with reagents that have been optimized for MS compatibility. The streamlined procedure enables digestion immediately after the immunoprecipitation elution step, facilitating MS sample preparation in ∼4 hours for same-day LC-MS/MS analysis. Applications: • Enrichment of low abundance targets to improve MS sensitivity • Identification of target proteins that are not detectable by western blotting • Quantitation of target proteins by LC-MS analysis • Screening of antibodies for IP-MS • Enrichment of target proteins upstream of targeted analysis (SRM)

Specifications

General

Description	Protein A/G Magnetic Beads
For Use With (Application)	MS-compatible immunoprecipitation
For Use With (Equipment)	Mass spectrometer
Kit Contents	Trypsin Protease, 2 x 20 μg<br />Trypsin Storage Solution, 250 μL<br />Protein A/G Magnetic Beads, 1 mL<br />IP-MS Cell Lysis Buffer, 100 mL<br />IP-MS Wash Buffer A, 75 mL<br />IP-MS Wash Buffer B, 40 mL<br />IP Elution and MS Sample Prep Buffer, 10 mL<br />Alkylation Solution, 1 mL/vial<br />Low Protein-Binding Collection Tubes, 50 x 1.5 mL<br />Bond-Breaker™ TCEP Solution, Neutral pH, 0.5 mL<br />10% Pierce™ Trifluoroacetic Acid (TFA), Sequencing Grade, 0.5 mL<br />1M MgCl<sub>2</sub>, 0.75 mL
Quantity	20 Reactions
Workflow Step	Sample prep enrichment
Detection Method	Mass Spectrometry
Product Line	SureQuant
Starting Material	Tissue
Unit Size	Each

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Thermo Fisher, A51743, SureQuant™ IP-MS Sample Preparation Kits

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