Thermo Fisher, A52021, Viral Production Cells 2.0 (CN)

Quantity: 1 mL vial
Unit Size: Each
• A clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System
• Cells have been adapted to the chemically defined Viral Production Medium and are maintained in suspension culture in that medium
• Viral Production Cells 2.0 (CN) are highly transfectable using the AAV-MAX Transfection Kit (Cat. No. A50515) for adeno-associated virus (AAV) production
• Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10^7viable cells/mL in Viral Production Medium and 10% DMSO
• Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX™ Supplement for optimal performance
• These cells are locally manufactured in ChinaFeatures of the Viral Production Cells 2.0 include:
• Clonal, 293F-derived, high-producer cell line
• Absence of SV40 large T antigen
• Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
• Enable production of high AAV titers, greater than 5x10^10vg/mL (unpurified)
• Robust scalability and passage stability