Thermo Fisher, A55124, MycoSEQ™ Plus Mycoplasma Detection Kits

Name: MycoSEQ™ Plus Mycoplasma Detection Kits
Brand: Thermo Fisher
SKU: A55124

Description: MycoSEQ™ Plus Mycoplasma Detection Kit, Unit Size: Each, The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples. Designed and tested using criteria often applied toward rapid mycoplasma detection in biotherapeutic manufacturing cell culture lot release, the MycoSEQ Plus kit enables results that meet or exceed guidance on sensitivity and specificity expectations as described in Europea

Catalog Number: A55124

Pricing

Send an RFQ.

View RFQ List

Category

Molecular Identification Kits

TagsPCR Equipment and Supplies PCR Supplies Real Time PCR Products Real Time PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Description: MycoSEQ™ Plus Mycoplasma Detection Kit
Unit Size: Each
This multiplexed assay enables predicted simultaneous screening of over 200 species of Mycoplasma target, discriminatory detection of an assay specific control template, and internal PCR control with no cross-reactivity to closely-related bacterial species.Features of the MycoSEQ Plus Mycoplasma Detection Kit• Provides results in less than 1 day• Includes a unique discriminatory positive control to differentiate a true positive result from a potential cross-contamination positive call• Includes protocols for various methods • Live mycoplasma is not needed: MycoSEQ Mycoplasma Detection Kit does not contain any live mycoplasma and does not require use of live mycoplasma for validation• Can be used with gDNA, inactivated mycoplasma or live mycoplasma• May be used as an alternative to the standard 28-day culture test• May be used in conjunction with culture-based methods by providing preliminary results while waiting for results from the 28-day testPrepSEQ nucleic acid extraction kits offer a robust and easy workflow to overcome complex matrix challenges to ensure the required sensitivity. Use MycoSEQ Plus Mycoplasma Detection Kit with PrepSEQ 1-2-3 Nucleic Acid Extraction Kit (Cat. No. A57925) for manual extractions, or MycoSEQ Plus Mycoplasma Detection Kit with PrepSEQ Express Nucleic Acid Extraction Kit (Cat. No. A57926) for automated extraction using the Automate Express Nucleic Acid Extraction System.Also available but not included with the detection kit: MycoSEQ gDNA kits (Cat. No. A49973 and A49976), containing purified genomic DNA from various mycoplasma species, may be used as positive test material during method development.Integrated workflow solutionThe MycoSEQ Plus Mycoplasma Detection Kit is part of an integrated workflow for adventitious agent, impurity and contaminant testing during biopharmaceutical manufacturing. The entire workflow including PrepSEQ Sample Preparation Kits, which offer manual or automated sample preparation approaches, together with qPCR analysis can provide results in under 5 hours. (Please note complex matrices may require additional upfront processing steps, as described in various available protocols.)MycoSEQ Plus kit performance has been tested and confirmed on the Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems QuantStudio 5 Real-Time PCR System. Data analysis using AccuSEQ Real-Time PCR Detection Software provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.

Specifications

General

Description	MycoSEQ™ Plus Mycoplasma Detection Kit
For Use With (Equipment)	QS5 0.1 mL Block or 7500 FAST, using sample preps PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit (Cat. No. 4452222), or PrepSEQ™ Express Nucleic Acid Extraction Kit (Cat. No. 4466351)
No. of Reactions	100 Reactions
Product Line	MycoSEQ
Product Type	Mycoplasma Detection
Quantity	100 reactions
Sample Type	Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures
Shipping Condition	Dry Ice
Target Organism Class	Mollicutes, mycoplasma
Detection Method	Primer-probe
For Use With (Application)	Microbial Detection
Form	Liquid
GC-Rich PCR Performance	Medium
Label or Dye	FAM
PCR Method	Multiplex qPCR
Reaction Speed	Standard
Unit Size	Each

FAQs

Question: What is the purpose of MycoSEQ Plus?

Answer:Real-time PCR detection of mycoplasma contamination.

Question: Is MycoSEQ Plus the same formulation as the original MycoSEQ kit?

Answer:No. Check the instructions for the specific Plus kit and workflow.

Question: Does the kit eliminate the need for validation?

Answer:No. Laboratories must establish suitability for their intended matrices and uses.

Application Note

MycoSEQ™ Plus Mycoplasma Detection Kit is a real-time PCR solution for detection of mycoplasma DNA in biopharmaceutical and cell-culture samples. It is designed for molecular contamination testing where laboratories need a defined assay with appropriate controls and sample preparation. The Plus kit supports a defined real-time PCR workflow for mycoplasma DNA testing. The appropriate PCR platform, extraction method, assay controls and sample matrix determine how the kit can be integrated into testing. Sample input, PCR controls and assay conditions form part of the validated testing workflow.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.