Description
Quantity: 1 Kit
Unit Size: Each
Features include:
• Optimized formulation for patient-derived tumoroid culture
• Streamlined media preparation, culture methods, and support
• Adaptable use for multiple cancer indications and downstream assaysOptimized performanceOncoPro Tumoroid Culture Medium is a novel, serum-free media system specifically developed for patient-derived cancer cell expansion. Over 40 conditions were tested during its development to identify the combination of basal medium, supplements, and growth factors that would best support long-term maintenance of donor-specific characteristics, including mutational profiles and gene expression patterns. The absence of Wnt-3A, R-spondin, Noggin, and small molecule inhibitors make OncoPro medium well-suited for complex biological studies and screening applications. Meanwhile, our strict ISO 13485 and cGMP quality standards help ensure that this ready-to-use media system provides consistency and reproducibility.Streamlined adoptionThis versatile media system includes a four-component kit that can be adapted for different cancer indications through addition of tissue-specific growth factors. An innovative suspension culture method eliminates the requirement for manual cell embedding in basement membrane extract domes, helping provide a cost-effective and less labor-intensive option for routine tumoroid culture. OncoPro medium is broadly compatible with various starting materials, including dissociated tumor cells (DTCs), patient-derived xenograft organoids (PDXOs), and tumoroid lines derived in other media systems. Comprehensive resources and support are available to help ensure effortless adoption.Adaptable applicationThe OncoPro Tumoroid Culture Medium Kit is a flexible system designed to help address complex research needs for long-term growth of tumoroid cell lines, and accommodates multiple cancer indications (colorectal, lung, pancreatic, head and neck, endometrial, breast) using a single media kit. Other epithelial cancer indications may also be supported. The kit’s scalable and automation-friendly suspension workflow facilitates smooth transitions to downstream research applications, including traditional 2D assays, diverse characterization techniques, and advanced cell-based modeling.
