Thermo Fisher, A57623, MegaScript™ T7 Transcription Kit Plus

Name: MegaScript™ T7 Transcription Kit Plus
Brand: Thermo Fisher
SKU: A57623

No. of Reactions: 1000 Reactions, Unit Size: Each, The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA. Each 20-μL reaction yields an average of 100 μg of RNA, while the scaled-up protocol yields up to 1 mg of RNA. This kit is suitable for full- or partially-modified-nucleotide substitution to produce modified RNA. It is also suitable for co-transcriptional capping with cap analogs to make capped RNA that can be

Catalog Number: A57623

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Category

Sequencing Reaction Kits

TagsBiochemical Reagents Transcription and Translation Reagents

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

No. of Reactions: 1000 Reactions
Unit Size: Each
Features of the MegaScript T7 Transcription Kit Plus include:
• Optimized to achieve high mRNA yields (>5 mg/mL)
• Flexible—allows use of modified nucleotides and/or cap analogs such as ARCA or CleanCap
• Includes GFP control template
• Made up of high quality reagents (TheraPure); eliminates the need to qualify new reagents during scale-up
• Available in two sizes: 50 and 1000 reactionsThe MegaScript T7 Transcription Kit Plus requires a linearized DNA template with a T7 RNA polymerase promoter to create >5 mg/mL of RNA. MEGAscript kits are intended for the synthesis of large amounts of unlabeled or low-specific-activity RNA for a variety of uses, including transfections, preclinical mRNA therapeutic mRNA studies, microinjections, in vitro translation, and RNA structure and function analysis. Note that this kit does not create capped RNA, which is necessary for translational studies. MEGAscript kits are not recommended for synthesis of high-specific-activity probes.

Specifications

General

No. of Reactions	1000 Reactions
Quantity	1000 Reactions
Cell Type	HEK293
Final Product Type	In vitro Transcribed RNA
Format	Kit
Isolation Technology	Silica Spin Column
Purification Target	In vitro Transcribed RNA
Sample Type	In vitro Transcribed RNA
Unit Size	Each

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Unless otherwise stated, the minimum order amount is USD 1,000.
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Thermo Fisher, A57623, MegaScript™ T7 Transcription Kit Plus

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