Thermo Fisher, A6222, ATTO-TAG™ CBQCA Derivatization Reagent (CBQCA; 3-(4-Carboxybenzoyl)quinoline-2-Carboxaldehyde)

Name: ATTO-TAG™ CBQCA Derivatization Reagent (CBQCA; 3-(4-Carboxybenzoyl)quinoline-2-Carboxaldehyde)
Brand: Thermo Fisher
SKU: A6222

Quantity: 10 mg, Unit Size: Each

Catalog Number: A6222

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Category

Fluorescent Dyes

Pack Size10 mg

TagsProtein Purification Protein Analysis Reagents Protein Purification Reagents and Kits

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 mg
Unit Size: Each
• High sensitivity, freedom from background and long-wavelength excitability make these potential reagents for researcher, diagnostic and forensic applications
• Resulting products of CBQCA are maximally excited at 450nm or by 442nm spectral line of He-Cd laser
• Have emission maxima at approx. 550nm
• In capillary zone electrophoresis (CZE), sensitivity of detection of ATTO-TAG CBQCA conjugates should be in attomole range (10-18 moles)Anion Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis

Specifications

General

Chemical Reactivity	Amine
For Use With (Application)	Cell Viability, Proliferation and Function; Protein Electrophoresis and Western Blotting
Label or Dye	ATTO-TAG CBQCA
Quantity	10 mg
Reactive Moiety	Aldehyde
Shipping Condition	Room Temperature
Target	Primary Amine
Form	Solid
Product Line	ATTO-TAG
Type	Derivatization Kits and Reagents
Unit Size	Each

FAQs

Question: What functional group does CBQCA react with?

Answer:CBQCA reacts with primary amines to form fluorescent derivatives.

Question: What fluorescence region is associated with CBQCA derivatives?

Answer:The derivatives can be excited near 450 nm and have emission maxima near 550 nm.

Question: How should the reagent be stored?

Answer:Store the solid reagent frozen at −5 to −30 °C and protect it from light.

Application Note

ATTO-TAG™ CBQCA is an amine-reactive derivatization reagent used to generate fluorescent conjugates from primary amines for sensitive electrophoretic and chromatographic analysis. CBQCA reacts with primary amines in peptides, proteins and other amine-containing compounds, while the derivatized products can be excited near 450 nm and emit near 550 nm. This long-wavelength fluorescence is useful for capillary electrophoresis and related separation workflows where low background and high detection sensitivity are important. The reagent is supplied as 10 mg of solid material. Store it frozen at −5 to −30 °C and protect it from light. Reaction conditions should be selected for the specific sample matrix and analytical method rather than transferred between unrelated labeling workflows.

Ordering

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