Thermo Fisher, AM1946, NorthernMax™-Gly Kit

Quantity: 1 kit
Unit Size: Each
• Use of glyoxal/DMSO instead of formaldehyde for sample preparation avoids the need to pour and run gels in a fume hood, and eliminates the safety issues associated with use of formaldehyde
• Glyoxal gels can be prepared at the lab bench: ;no noxious reagents
• Increased sensitivity up to 100x over standard hybridization protocols
• Reduce hybridization to 2 hours for many messages
• Complete kit contains ULTRAhyb™ Hybridization Buffer and gel and wash reagents for 10 to 20 gels
• Compatible with RNA, DNA, or oligonucleotide probes labeled isotopically or nonisotopically
• Sufficient reagents are provided for processing 1000cm^2membrane
• RNA samples denatured with glyoxal may show sharper bands on northern blots compared to samples denatured and run in the presence of formaldehyde
• The streamlined NorthernMax-Gly procedure is more convenient and faster than the standard glyoxal gel protocol: A single reagent is used for sample denaturation and loading, the incubation time for sample denaturation has been reduced, and no recirculation of buffer is required during electrophoresis
• The volume ratio of glyoxal denaturation solution to sample RNA is lower than many protocols, so that sample precipitation prior to gel loading is usually not required
• An RNA sample and template for probe synthesis are also provided as controls for each kit
• Compatible with glyoxal-containing gelsDNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting