Thermo Fisher, AMQAF2000, Countess™ 3 FL Automated Cell Counter

Name: Countess™ 3 FL Automated Cell Counter
Brand: Thermo Fisher
SKU: AMQAF2000

Type: Automated Cell Counter, Warranty: 1-year Standard, Software: Countess 3/3 FL Cell Counter Software, Unit Size: Each, The Countess 3 FL Automated Cell Counter is a benchtop brightfield and fluorescence cell counter with advanced optics, automated focus and lighting, and image analysis software driven by a neural network-based machine-learning algorithm for rapid automated cell counting of primary and immortalized cell lines. The Countess 3 FL counter allows brightfield or fluorescence cell

Catalog Number: AMQAF2000

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Category

Cell Counters

TagsCell Analysis Products Cellular Imaging Cellular Imaging Instrumentation

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Type: Automated Cell Counter
Warranty: 1-year Standard
Software: Countess 3/3 FL Cell Counter Software
Unit Size: Each
Features of the Countess 3 FL Automated Cell Counter include:
• Accurate—highly accurate machine-learning algorithm applied to automated cell counting, eliminates the subjectivity of manual cell counting and user-to-user variability
• Flexible—accepts up to two EVOS light cubes, user selectable from fifteen fluorescent channels; compatible with both disposable and reusable cell counting chamber slides
• Fast—counts live and dead cells, measures viability, reports average cell size, calculates percent cell aggregates, and saves data with a single touch, in about 20 seconds
• Convenient—requires no cleaning, routine maintenance, or calibration; high-resolution capacitive touchscreen and simple user interface provide quick startup and requires minimal training
• Versatile—perform automated counting of single cells and isolated nuclei for RNA sequencing and gene expression applications in single-cell analysis workflowsThe Countess 3 FL Automated Cell Counter works in two simple steps:
• Mix 10 μL of sample with 10 μL of trypan blue or other desired reagent, including ReadyCount stain, and pipet into a disposable or reusable Countess chamber slide.
• Insert the slide into the instrument. Rapid Capture and Auto-Save modes allow automated cell counting with a single touch. Focus, lighting, and file saving are executed automatically.Obtain accurate cell countsThe Countess 3 FL Automated Cell Counter uses trypan blue staining combined with auto-focus and lighting driven by a machine-learning image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 x 10^4 to 1 x 10^7 cells/mL (the optimal range is 1 x 10^5 to 4 x 10^6 cells/mL), which is broader than that of a hemocytometer.Simple operation with fluorescent application versatilityOperated using an intuitive user interface, automated cell counting with the Countess 3 FL counter gives you the data you need to prepare your sample for the next passage or experiment and allows you to multiplex with a variety of fluorescent dyes and proteins excited and detected via EVOS light cubes. With both brightfield and fluorescence cell counting options, you can perform cell counts for routine cell culture applications along with more extensive fluorescence analysis of cells such as live vs. dead, nucleated vs. non-nucleated, and transfected vs. untransfected. You no longer need complex, expensive instruments to obtain reliable data.Transfer data and images to your PC or cloudUsing the USB port on the Countess 3 FL Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. For your convenience, a USB drive and WiFi dongle are supplied with the Countess 3 FL instrument.Improve downstream single-cell analysisEnsure accurate single-cell analysis and RNA sequencing results by minimizing cell aggregates and obtaining precise viable cell counts. For optimal single-cell data output, use the percent cell aggregates on the Countess 3 FL to confirm <5% aggregates in the starting sample before single-cell analysis. Staining with fluorescent dyes such as the ReadyCount stains and using the Countess 3 FL-based count mode offers more accurate counts and viability measurements for samples with debris or out-of-focus cells in brightfield. The Countess 3 FL and ReadyCount stains are also recommended for counting isolated nuclei in scATAC-Seq studies. View the single cell and nuclei counting application note for more details.The Countess 3 FL Automated Cell Counter can be purchased as the instrument (Cat. No. AMQAF2000) or a variety of packages bundled with additional accessories, extended warranties, or software, such as the Countess 3 FL Starter Package (Cat. No. A49866), which includes:
• Countess FL Automated Cell Counter
• EVOS GFP and TexasRed light cubes
• Reusable slide
• 500 disposable slides with trypan blueExtended warranty instrument and starter packagesThe Countess 3 FL Automated Cell Counter comes with a standard 1-year warranty that covers all costs for shipping, parts, and labor for repairs. Service plans can be bundled with new instrument purchases in an extended warranty package that extends the standard warranty by 2 years for a total of 3. Additional years of coverage can also be purchased to extend the standard 1-year or extended 3-year warranty. The Countess 3 FL instrument and starter packages are offered with two different types of bundled extended warranty packages:
• 3-year Rapid Exchange (REX) Extended Warranty(1 year standard warranty + 2 years Rapid Exchange, 3 years total exchange entitlement)Replacement shipped out in 1 business day for labs with critical timelinesAvailable with the instrument only (Cat. No. A49892) and the Countess 3 FL Starter Package (Cat. No. A50299)
• 3-year AB Repair Center (ABRC) Extended Warranty(1 year standard warranty + 2 years extended repair coverage)Instrument repaired and returned in ~3 weeks for regulated labs required to maintain asset tagging consistency (loaner instrument shipped within 1 business day with upgrade to an ABRC Support Plus contract)Available with the instrument only (Cat. No. A49893) and the Countess 3 FL Starter Package (Cat. No. A50303)Countess 3 FL with 21 CFR Part 11 Software Module (Cat. No. AMQAF2001)The included Countess 3 21 CFR Part 11 Software Module is for use with the Countess 3 and Countess 3 FL automated cell counters and supports compliance with 21 CFR Part 11 FDA guidelines for security, auditing, and electronic (SAE) signatures using an SAE administration console. The 21 CFR Part 11 software can also be purchased separately (Cat. No. A51025).

Specifications

General

Certifications/Compliance	RoHS
Detection Method	Brightfield, Fluorescence
Dimensions	9 x 9 x 5.5 in. (228.6 x 228.6 x 139.7 mm)
Display	LCD (1280 x 800 pixels)
For Use With (Application)	Cell Culture and Analysis Workflows
For Use With (Equipment)	EVOS Microscopes, Countess Chamber Slides, and Reusable Slide
Frequency	50/60 Hz
Holds	2 samples per slide
Magnification	2.5X
Memory	Onboard, USB drive
Optics	3 Channels (Brightfield and 2 slots for EVOS™ LED light cubes)
Quantity	1 instrument
Shipping Condition	Ambient
Software	Countess 3/3 FL Cell Counter Software
Voltage	100/240 V
Warranty	1-year Standard
Weight (English)	8 lb.
Weight (Metric)	3.6 kg
Camera	5 MP
Product Line	Countess
Type	Automated Cell Counter
Unit Size	Each

FAQs

Question: What concentration range can the Countess 3 FL measure?

Answer:The measurement range is 1 × 10^4 to 1 × 10^7 cells/mL, with an optimal range of 1 × 10^5 to 4 × 10^6 cells/mL.

Question: Can it perform fluorescence-based counting?

Answer:Yes. It supports brightfield and two optional fluorescence channels and accepts up to two EVOS light cubes selected from fifteen fluorescent channels.

Question: Which chamber slides are compatible?

Answer:The instrument works with both disposable and reusable Countess cell-counting chamber slides.

Application Note

Countess 3 FL Automated Cell Counter is used for rapid brightfield and fluorescence counting of cells and isolated nuclei, fluorescence monitoring, apoptosis assessment, and cell-viability measurement. The benchtop instrument combines advanced optics, automated focus and lighting, and neural-network-based image analysis to reduce the subjectivity and user-to-user variability associated with manual counting. It provides brightfield plus two optional fluorescence channels and accepts up to two EVOS light cubes selected from fifteen fluorescent channels. This flexibility supports routine cell-culture counts as well as live/dead, nucleated/non-nucleated, and transfected/untransfected analyses. A standard workflow mixes 10 µL of sample with 10 µL of trypan blue or another chosen reagent, loads the mixture into a disposable or reusable Countess chamber slide, and inserts the slide. Rapid Capture and Auto-Save automate focus, lighting, counting, and file saving. In about 20 seconds, the instrument reports live and dead cell counts, viability, average cell size, and the percentage of cell aggregates. Its measurement range is 1 × 10^4 to 1 × 10^7 cells/mL, with an optimal range of 1 × 10^5 to 4 × 10^6 cells/mL. For single-cell analysis, RNA sequencing, and gene-expression workflows, it can quantify isolated nuclei and help confirm that the starting sample contains less than 5% aggregates. Results and images transfer through USB, while the touchscreen interface, no-cleaning workflow, and absence of routine maintenance or calibration simplify daily operation.

Ordering

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