Thermo Fisher, C10427, CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit, 100 Assays
Quantity: 100 Assays, Unit Size: Each
Catalog Number: C10427
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Category
Pack Size100 assays
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| Excitation/Emission | SYTOX AADvanced: 546/647<br />CellEvent Caspase 3/7 Green Detection Reagent: 511/533 |
|---|---|
| Flow Cytometer Laser Lines | 488 |
| For Use With (Application) | Flow Cytometry |
| For Use With (Equipment) | Attune™ Acoustic Focusing Cytometer, Flow Cytometer |
| Label Type | Other Label(s) or Dye(s) |
| Label or Dye | CellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX AADvanced dead cell stain |
| Product Line | CellEvent |
| Product Type | Caspase Assay Kit |
| Quantity | 100 Assays |
| Shipping Condition | Wet Ice |
| Solubility | DMSO (Dimethylsulfoxide) |
| Detection Method | Fluorescence |
| Format | Kit |
| Unit Size | Each |
Answer:It detects activation of caspase-3 and caspase-7, key executioner enzymes in apoptosis.
Answer:It identifies cells with compromised plasma membranes so dead cells can be distinguished from viable and apoptotic populations.
Answer:The kit provides reagents for 100 assays.
CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit is used to detect apoptosis by measuring activation of executioner caspases 3 and 7 in individual cells. The CellEvent reagent contains a DEVD peptide linked to a nucleic-acid-binding fluorogenic dye. In apoptotic cells, activated caspase-3 or caspase-7 cleaves the DEVD sequence, releasing the dye so it can bind DNA and generate a bright green fluorescence signal. The kit also includes SYTOX™ AADvanced dead-cell stain, allowing viable, apoptotic, and membrane-compromised cells to be distinguished in a single flow-cytometry workflow. The assay can be excited with a 488 nm laser and does not require fixation or washing before acquisition. This 100-assay configuration supports apoptosis studies, drug-response testing, and cell-death characterization in suspension or dissociated cell samples.
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